概述

  • 产品名称Anti-Hsp90 alpha抗体
    参阅全部 Hsp90 alpha 一抗
  • 描述
    兔多克隆抗体to Hsp90 alpha
  • 特异性ab74248 weakly cross reacts with Hsp90 beta
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Pig, Chimpanzee, Cynomolgus Monkey, Chinese Hamster
  • 免疫原

    Synthetic peptide corresponding to Mouse Hsp90 alpha aa 2-12 (N terminal).
    Sequence:

    PEETQTQDQPM-Cys

  • 阳性对照
    • MAD109 cells or breast carcinoma

性能

应用

Our Abpromise guarantee covers the use of ab74248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 85 kDa.

Use at a concentration of 5 - 10 µg/ml for 2 hours at room temperature.

IP Use at 10 µg/mg of lysate.

(Native verified, use Protein A)

IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at a concentration of 5 - 10 µg/ml for 30 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 to 20 minutes followed by cooling at room temperature for 20 minutes.

ICC/IF Use at an assay dependent concentration.

靶标

Anti-Hsp90 alpha antibody 图像

  • Formalin fixed, paraffin embedded human breast carcinoma tissue stained with ab74248 at 10µg/ml, using peroxidase conjugate and AEC chromogen. Note cytoplasmic and nuclear staining of tumor cells.
  • Anti-Hsp90 alpha antibody (ab74248) at 10 µg/ml + Mouse cells-1

    Predicted band size : 85 kDa
    Observed band size : 85 kDa
  • IP/Western blot of mouse cells-1. IP with ab74248 at 10µg/mg protein lysates.

Anti-Hsp90 alpha antibody (ab74248)参考文献

This product has been referenced in:
  • Wang H  et al. Hsp90a forms a stable complex at the cilium neck for the interaction of signalling molecules in IGF-1 receptor signalling. J Cell Sci 128:100-8 (2015). Read more (PubMed: 25359884) »

See 1 Publication for this product

Product Wall

We do carry a number of control lysates and tissue slides including those from breast tumor carcinoma. I have included links to these below. Breast tumor (human): ductal carcinoma (invasive) tissue slides (ab4697): http://www.abcam.com/breast-tumor-...

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Thank you for your reply. I can have ab79849 shipped to you next week but I want to make sure that there will be someone there to receive it. As it is being shipped to , we cannot guarantee a delivery date as there is a chance that it could get...

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Thank you for your reply. I am sorry about the problems that you are having with ab74248 in western blot. I would be happy to send you a replacement antibody, a few possible alternatives are: http://www.abcam.com/Hsp90-alpha-antib...

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Thank you for contacting Abcam. I am sorry that you are having issues with ab74248 in western blot. We do guarantee that this antibody will work in western blot in mouse, rat and human cells. I was wondering if you could please tell me which type of ce...

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Thank you for attending our webinar on Immunoprecipitation last week. We hope you enjoyed it and it was useful for you. We would also like to thank you for sending your question and apologize that we were not able to answer it in the Q&A session. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"