Anti-Hsp90 alpha抗体(ab53110)

概述

  • 产品名称Anti-Hsp90 alpha抗体
    参阅全部 Hsp90 alpha 一抗
  • 描述
    兔多克隆抗体to Hsp90 alpha
  • 特异性ab53110 detects endogenous levels of total Hsp90 protein.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    A synthetic peptide derived from human Hsp90 alpha.

  • 阳性对照
    • Extracts from NIH/3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab53110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/300 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Molecular chaperone. Has ATPase activity.
  • 序列相似性Belongs to the heat shock protein 90 family.
  • 翻译后修饰ISGylated.
  • 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EL52 antibody
    • epididymis luminal secretory protein 52 antibody
    • Heat shock 86 kDa antibody
    • heat shock 90kD protein 1, alpha antibody
    • Heat shock 90kD protein 1, alpha like 4 antibody
    • heat shock 90kD protein, alpha-like 4 antibody
    • Heat shock 90kDa protein 1 alpha antibody
    • Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody
    • Heat shock protein HSP 90-alpha antibody
    • HS90A_HUMAN antibody
    • HSP 86 antibody
    • HSP86 antibody
    • Hsp89 antibody
    • HSP89A antibody
    • Hsp90 antibody
    • HSP90A antibody
    • HSP90AA1 antibody
    • HSP90ALPHA antibody
    • HSP90N antibody
    • HSPC1 antibody
    • HSPCA antibody
    • HSPCAL1 antibody
    • HSPCAL4 antibody
    • HSPN antibody
    • LAP 2 antibody
    • LAP2 antibody
    • lipopolysaccharide-associated protein 2 antibody
    • LPS-associated protein 2 antibody
    • Renal carcinoma antigen NY-REN-38 antibody
    see all

Anti-Hsp90 alpha antibody 图像

  • All lanes : Anti-Hsp90 alpha antibody (ab53110) at 1/300 dilution

    Lane 1 : extract from NIH/3T3 cells
    Lane 2 : extract from NIH/3T3 cells with immunising peptide


    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • Ab53110 staining human normal placenta tissue. Staining is localised to cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Hsp90 alpha antibody (ab53110)参考文献

ab53110 has not yet been referenced specifically in any publications.

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