Anti-Hsp90 alpha抗体[2G5.G3] (ab79849)

概述

  • 产品名称Anti-Hsp90 alpha抗体[2G5.G3]
    参阅全部 Hsp90 alpha 一抗
  • 描述
    小鼠单克隆抗体[2G5.G3] to Hsp90 alpha
  • 特异性ab79849 is Hsp90 alpha specific (>96% alpha specific by ELISA).
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IHC-P, ELISA, WB, IP, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Human Hsp90 alpha

  • 阳性对照
    • Heat shocked HeLa cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human Testis.

性能

应用

Our Abpromise guarantee covers the use of ab79849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 85 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Molecular chaperone. Has ATPase activity.
  • 序列相似性Belongs to the heat shock protein 90 family.
  • 翻译后修饰ISGylated.
  • 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EL52 antibody
    • epididymis luminal secretory protein 52 antibody
    • Heat shock 86 kDa antibody
    • heat shock 90kD protein 1, alpha antibody
    • Heat shock 90kD protein 1, alpha like 4 antibody
    • heat shock 90kD protein, alpha-like 4 antibody
    • Heat shock 90kDa protein 1 alpha antibody
    • Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody
    • Heat shock protein HSP 90-alpha antibody
    • HS90A_HUMAN antibody
    • HSP 86 antibody
    • HSP86 antibody
    • Hsp89 antibody
    • HSP89A antibody
    • Hsp90 antibody
    • HSP90A antibody
    • HSP90AA1 antibody
    • HSP90ALPHA antibody
    • HSP90N antibody
    • HSPC1 antibody
    • HSPCA antibody
    • HSPCAL1 antibody
    • HSPCAL4 antibody
    • HSPN antibody
    • LAP 2 antibody
    • LAP2 antibody
    • lipopolysaccharide-associated protein 2 antibody
    • LPS-associated protein 2 antibody
    • Renal carcinoma antigen NY-REN-38 antibody
    see all

Anti-Hsp90 alpha antibody [2G5.G3] 图像

  • IHC image of Hsp90 alpha staining in Human Testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79849, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab79849 at 1/100 dilution staining Hsp90 alpha in human keratinocyte cell line HaCaT by Immunocytochemistry/ immunofluorescence. A Fluorophore conjugated goat anti mouse was used as secondary at 1/50 dilution.

  • Overlay histogram showing HeLa cells stained with ab79849 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79849, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Hsp90 alpha antibody [2G5.G3] (ab79849)参考文献

This product has been referenced in:
  • Al-Alwan LA  et al. CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines. J Immunol 193:1416-26 (2014). Read more (PubMed: 24981451) »
  • El-Kasaby A  et al. A cytosolic relay of heat shock proteins HSP70-1A and HSP90ß monitors the folding trajectory of the serotonin transporter. J Biol Chem 289:28987-9000 (2014). Read more (PubMed: 25202009) »

See all 4 Publications for this product

Product Wall

Thank you for yoru recent telephone enquiry.

I ampleased to let you know I now have the ELISA testing protocol for ab79849 -Hsp90 alpha antibodyfrom the originator. Thank you for your patience.
I have copied this below:

Purif...

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Thank you for your reply. I will forward your request to 51AB, who will be in contact with you to process your refund request. I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

Thank you for your reply with this information. It seems that the lower band is certainly isoform 1 of HSP90 and it is likely that the higher band is isoform2. I am not an expert in this field and could not say for sure if both isoforms are express...

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Thank you for contacting Abcam regarding ab79849. I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the data provided and would like to point out that there is a second isoform of this protein that is ...

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Than you for your inquiry and I am sorry to hear that you are experiencing some trouble with ab53497 and ab79849. Are you running any isotype controls with these experiments? I just want to make sure that the pull down with the HBP21 antibody i...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (primary cancer-associated fibroblasts (prostate))
Loading amount 30 µg
Specification primary cancer-associated fibroblasts (prostate)
Gel Running Conditions Reduced Denaturing (Invitrogen's NuPAGE 4-12%)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Alexander Henke

Verified customer

提交于 Aug 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"