Anti-Hsp75抗体[1A1] (ab119398)

概述

  • 产品名称Anti-Hsp75抗体[1A1]
    参阅全部 Hsp75 一抗
  • 描述
    小鼠单克隆抗体[1A1] to Hsp75
  • 经测试应用适用于: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Monkey
  • 免疫原

    Recombinant full length protein of Human Hsp75 produced in HEK293T cells (NP_057376).

  • 阳性对照
    • HEK293T cells transfected with Hsp75, HepG2, heLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, and MCF7 extracts, Carcinoma of Human pancreas, Human endometrium, Adenocarcinoma of Human endometrium, and Carcinoma of Human bladder tissues, COS7 cells transiently transfected with Hsp75
  • 常规说明Dilute in PBS (pH7.3) before use.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号1A1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab119398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Predicted molecular weight: 80 kDa.
IHC-P 1/150.
ICC/IF 1/100.

靶标

  • 功能Chaperone that expresses an ATPase activity.
  • 组织特异性Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
  • 序列相似性Belongs to the heat shock protein 90 family.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heat Shock Protein 75 antibody
    • Heat shock protein 75 kDa antibody
    • Heat shock protein 75 kDa mitochondrial antibody
    • HSP 75 antibody
    • HSP90L antibody
    • mitochondrial antibody
    • TNF receptor associated protein 1 antibody
    • TNFR Associated Protein antibody
    • TNFR-associated protein 1 antibody
    • TRAP-1 antibody
    • Trap1 antibody
    • TRAP1_HUMAN antibody
    • Tumor Necrosis Factor Receptor Associated Protein 1 antibody
    • Tumor necrosis factor type 1 receptor associated protein antibody
    • Tumor necrosis factor type 1 receptor-associated protein antibody
    see all

Anti-Hsp75 antibody [1A1] 图像

  • Overlay histogram showing HepG2 cells stained with ab119398 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119398, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-Hsp75 antibody [1A1] (ab119398) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Hsp75

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 80 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203439)
  • All lanes : Anti-Hsp75 antibody [1A1] (ab119398) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 80 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203439)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using ab119398 at a dilution of 1/150.
  • mmunohistochemical staining of paraffin-embedded Human endometrium tissue using ab119398 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using ab119398 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using ab119398 at a dilution of 1/150.
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp75 using ab119398 at a dilution of 1/100.

Anti-Hsp75 antibody [1A1] (ab119398)参考文献

ab119398 has not yet been referenced specifically in any publications.

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