The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). (ECL).
功能In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
组织特异性HSPA1B is testis-specific.
序列相似性Belongs to the heat shock protein 70 family.
细胞定位Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Western blot - Anti-Hsp70 antibody [C96F3-3] (ab115651)
All lanes : Anti-Hsp70 antibody [C96F3-3] (ab115651) at 1/1000 dilution
Lane 1 : HSP70 Recombinant Human Protein Lane 2 : HSP70B’ Recombinant Human Protein Lane 3 : HSP70-A1 Recombinant Mouse Protein Lane 4 : HSP70 Recombinant Chinook Salmon Protein Lane 5 : HSC70 Recombinant Bovine Protein Lane 6 : HSC70 (ATPase Fragment) Recombinant Bovine Protein Lane 7 : HeLa Cell Lysate Lane 8 : HeLa Cell Lysate, Heat Shocked Lane 9 : 3T3 Cell Lysate Lane 10 : 3T3 Cell Lysate, Heat Shocked Lane 11 : PC-12 Cell Lysate Lane 12 : PC-12 Cell Lysate, Heat Shocked
Predicted band size : 70 kDa
Anti-Hsp70 antibody [C96F3-3] (ab115651)参考文献
has not yet been referenced specifically in any publications.