Anti-Hsp70抗体(ab79852)
Key features and details
- Rabbit polyclonal to Hsp70
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: n/a
概述
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产品名称
Anti-Hsp70抗体
参阅全部 Hsp70 一抗 -
描述
兔多克隆抗体to Hsp70 -
宿主
Rabbit -
特异性
Detects a 70kDa protein corresponding to the molecular mass of inducible hsp70. May cross-react with Hsc70 at lower dilutions. -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Macaque monkey, African green monkey, Chinese hamster, Orangutan, Bos mutus grunniens, Saguinus oedipus -
免疫原
Recombinant full length protein corresponding to Human Hsp70 aa 1 to the C-terminus.
Database link: P0DMV8/P0DMV9 -
阳性对照
- IHC-P: mouse Inflamed colon tissue; human colon carcinoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
研究领域
相关产品
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Compatible Secondaries
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79852于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (2) |
1/100.
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WB | (1) |
1/10000. Detects a band of approximately 70 kDa.
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IHC-P |
1/100 - 1/1000.
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说明 |
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ICC/IF
1/100. |
WB
1/10000. Detects a band of approximately 70 kDa. |
IHC-P
1/100 - 1/1000. |
靶标
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相关性
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
细胞定位
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. -
数据库链接
- Entrez Gene: 281825 Cow
- Entrez Gene: 403612 Dog
- Entrez Gene: 15511 Human
- Entrez Gene: 3303 Human
- Entrez Gene: 193740 Mouse
- Entrez Gene: 3304 Mouse
- Entrez Gene: 396906 Pig
- Entrez Gene: 24472 Rat
see all -
别名
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
see all
图片
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All lanes : Anti-Hsp70 antibody (ab79852) at 1/25000 dilution
Lane 1 : Cell lysates prepared from mouse Pam212 cells
Lane 2 : Molecular weight marker -
Immunohistochemistry of human colon carcinoma staining Hsp70 using ab79852 at 1:50000. A Biotin conjugated Goat Anti-Rabbit antibody at 1:2000 was used as a secondary antibody and Methyl Green at 200uL was used to counter stain.
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Immunohistochemical analysis of paraffin-embedded formalin fixed (4%) mouse Inflamed colon tissue labelling Hsp70 with ab79852 at 1/1000 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (Biotin) at 1/2000 dilution for 1 hour at RT. Counter stained with Methyl Green at 200uL for 2 min at RT.
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Immunocytochemistry/Immunofluorescence analysis of heat shocked HeLa cells staining Hsp70 using ab79852 at a 1:100 dilution. The secondary antibody was a FITC conjugated Goat Anti-Rabbit (green) at a 1:200 dilution. Counterstain: DAPI (blue) nuclear stain at 1:40000. A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite.
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ab79852 staining Hsp70 in mouse backskin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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All lanes : Anti-Hsp70 antibody (ab79852) at 1/25000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from human A431 cells
Lane 3 : Cell lysates prepared from human A549 cells
Lane 4 : Cell lysates prepared from human HCT116 cells
Lane 5 : Cell lysates prepared from Hela cells
Lane 6 : Cell lysates prepared from HEK293 cells
Lane 7 : Cell lysates prepared from HepG2 cells
Lane 8 : Cell lysates prepared from HL-60 cells
Lane 9 : Cell lysates prepared from HUVEC cells
Lane 10 : Cell lysates prepared from Jurkat cells
Lane 11 : Cell lysates prepared from MCF7 cells
Lane 12 : Cell lysates prepared from PC3 cells
Lane 13 : Cell lysates prepared from T98G cells
Lane 14 : Tissue lysates prepared from Rat brain
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (52)
ab79852 被引用在 52 文献中.
- Panachan J et al. Extracellular Vesicle-Based Method for Detecting MYCN Amplification Status of Pediatric Neuroblastoma. Cancers (Basel) 14:N/A (2022). PubMed: 35681607
- Song C et al. HSP70 alleviates sepsis-induced cardiomyopathy by attenuating mitochondrial dysfunction-initiated NLRP3 inflammasome-mediated pyroptosis in cardiomyocytes. Burns Trauma 10:tkac043 (2022). PubMed: 36439706
- Uchida A et al. SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse. Nat Commun 13:7860 (2022). PubMed: 36543770
- Yang C et al. Exosome miR-134-5p restrains breast cancer progression via regulating PI3K/AKT pathway by targeting ARHGAP1. J Obstet Gynaecol Res 47:4037-4048 (2021). PubMed: 34378285
- Parry HA et al. Short and long-term effect of reproduction on mitochondrial dynamics and autophagy in rats. Heliyon 7:e08070 (2021). PubMed: 34622072