Anti-Hsp70抗体(ab66262)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to Hsp70
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Caenorhabditis elegans, Zebrafish
    预测可用于: Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Pig, African green monkey
  • 免疫原

    A region within synthetic peptide: SLFEGIDFYT SITRARFEEL CSDLFRSTLE PVEKALRDAK LDKAQIHDLV, corresponding to amino acids 286-335 of Human Hsp70

  • 阳性对照
    • Liver lysate.

性能

应用

Our Abpromise guarantee covers the use of ab66262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 70 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/312500.

靶标

图片

  • Anti-Hsp70 antibody (ab66262) at 1.25 µg/ml + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab66262 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Loading amount
20 µg
Specification
Lung
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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提交于 Dec 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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