概述

  • 产品名称Anti-Hsp70抗体
    参阅全部 Hsp70 一抗
  • 描述
    兔多克隆抗体to Hsp70
  • 经测试应用适用于: IHC-P, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    CQEVISWLDANTLAEKDE

    , corresponding to amino acids 574-591 of Human Hsp70

  • 阳性对照
    • Hela cells. Breast cancer.

性能

应用

Our Abpromise guarantee covers the use of ab31010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
IP
WB
  • 应用说明IHC-P: 1/100 for 30 min at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM
    citrate buffer, pH 6.0 for 10 min and then cool at RT for 20 min.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 70 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Hsp70 antibody 图像

    • Hsp70 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Hsp70 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

      The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31010.

      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

      Band: 70kDa; Hsp70

    • Tissue: Human breast cancer (formalin fixed/paraffin).
      Staining: ab31010 at 1/100 dilution for 30 minutes at room temperature.
    • Anti-Hsp70 antibody (ab31010) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 70 kDa
      Observed band size : 72 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 76 kDa (possible post-translational modification).
    • Anti-Hsp70 antibody (ab31010) at 1/25 dilution + 293 cell lysate

      Predicted band size : 70 kDa
      Observed band size : 70 kDa

    Anti-Hsp70 antibody (ab31010)参考文献

    This product has been referenced in:
    • Madrigal-Matute J  et al. Heat shock protein 90 inhibitors attenuate inflammatory responses in atherosclerosis. Cardiovasc Res 86:330-7 (2010). WB, IHC-P ; Mouse . Read more (PubMed: 20154064) »
    • Chu Y  et al. Alterations in lysosomal and proteasomal markers in Parkinson's disease: relationship to alpha-synuclein inclusions. Neurobiol Dis 35:385-98 (2009). Read more (PubMed: 19505575) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions Reduced Denaturing (10%)
    Loading amount 20 µg
    Specification Skeletal muscle
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 09 2016

    Thank you for calling Abcam earlier today.

    I have talked to the lab and ab31010 was raised against a synthetic peptide corresponding to The IHC and WB data both show results appropriate for HSP-70-1/2, thus we would expect that you should see...

    Read More

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Wir haben keinen Antikörper gegen HSP70 oder 90 im Katalog, der schon für Aspergillus terreus getestet ist.

    Ich habe die Immunogene unserer ...

    Read More

    Thank you for your enquiry. The following reference reports HSP70 in the nuclei of cells at the onset of caspase activation: Nature. 2007 Jan 4;445(7123):102-5. Epub 2006 Dec 10. PMID: 17167422 and this reference describes the subcellular l...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"