Anti-Hsp70抗体[4E7] (ab48515)

概述

  • 产品名称Anti-Hsp70抗体[4E7]
    参阅全部 Hsp70 一抗
  • 描述
    小鼠单克隆抗体[4E7] to Hsp70
  • 特异性This antibody is specific for Hsp70
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-641 of Human Hsp70, purified from E. coli

  • 阳性对照
    • HeLa cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 10% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
  • 克隆单克隆
  • 克隆编号4E7
  • 骨髓瘤Sp2/0
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab48515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100.
WB 1/1000 - 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ELISA Use at an assay dependent concentration.

靶标

  • 功能In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
  • 组织特异性HSPA1B is testis-specific.
  • 序列相似性Belongs to the heat shock protein 70 family.
  • 细胞定位Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DnaK type molecular chaperone HSP70 1 antibody
    • Epididymis secretory protein Li 103 antibody
    • FLJ54303 antibody
    • FLJ54370 antibody
    • FLJ54392 antibody
    • FLJ54408 antibody
    • FLJ75127 antibody
    • Heat shock 70 kDa protein 1 antibody
    • Heat shock 70 kDa protein 1/2 antibody
    • Heat shock 70 kDa protein 1A/1B antibody
    • Heat shock 70kDa protein 1A antibody
    • Heat shock 70kDa protein 1B antibody
    • Heat shock induced protein antibody
    • HEL S 103 antibody
    • HSP70 1 antibody
    • HSP70 1B antibody
    • HSP70 2 antibody
    • HSP70-1/HSP70-2 antibody
    • HSP70-1A antibody
    • HSP70.1 antibody
    • HSP70.1/HSP70.2 antibody
    • HSP70I antibody
    • HSP71_HUMAN antibody
    • HSP72 antibody
    • HSPA1 antibody
    • HSPA1A antibody
    • HSPA1B antibody
    see all

Anti-Hsp70 antibody [4E7] 图像

  • Anti-Hsp70 antibody [4E7] (ab48515) at 1/1000 dilution + HeLa cell lysate (30µg)

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
  • ab48515 staining Hsp70 in human eccrine gland section, by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/100 dilution and incubated with sample for 2 hours at 24°C. The DAB detection system was used with DAB chromogen.

Anti-Hsp70 antibody [4E7] (ab48515)参考文献

ab48515 has not yet been referenced specifically in any publications.

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