概述

  • 产品名称Anti-Hsp60抗体[Mab11-13]
    参阅全部 Hsp60 一抗
  • 描述
    小鼠单克隆抗体[Mab11-13] to Hsp60
  • 经测试应用适用于: WB, IP, ICC, Flow Cyt, Electron Microscopy, IHC-Fr, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Pig, Drosophila melanogaster, Monkey, Snake, Dolphin, Rainbow Trout
    不与反应: Saccharomyces cerevisiae, Escherichia coli
  • 免疫原

    Recombinant fragment, amino acids 31-547 (Human).

  • 表位This antibody recognizes a surface epitope of Hsp60 in the region of amino acids 288-366.
  • 阳性对照
    • HeLa Heat Shocked Cell Lysate or Recombinant Human Hsp60 Protein IF/ICC: HeLa cell line.

性能

应用

Our Abpromise guarantee covers the use of ab13532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 68.8 kDa).
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Electron Microscopy Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • 疾病相关Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • 序列相似性Belongs to the chaperonin (HSP60) family.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all

Anti-Hsp60 antibody [Mab11-13] 图像

  • ICC/IF image of ab13532 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13532, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab13532 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13532, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Hsp60 antibody [Mab11-13] (ab13532)参考文献

This product has been referenced in:
  • Lu Z  et al. B-Cell Epitopes in GroEL of Francisella tularensis. PLoS One 9:e99847 (2014). WB . Read more (PubMed: 24968190) »
  • Pathak SK  et al. Activated Apoptotic Cells Induce Dendritic Cell Maturation via Engagement of Toll-like Receptor 4 (TLR4), Dendritic Cell-specific Intercellular Adhesion Molecule 3 (ICAM-3)-grabbing Nonintegrin (DC-SIGN), and ß2 Integrins. J Biol Chem 287:13731-42 (2012). WB, Neutralising ; Human . Read more (PubMed: 22396536) »

See all 6 Publications for this product

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This antibody goes only by volume even though it is purified protein. The amount is proprietary information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"