概述

  • 产品名称Anti-Hsp60抗体[LK-2]
    参阅全部 Hsp60 一抗
  • 描述
    小鼠单克隆抗体[LK-2] to Hsp60
  • 经测试应用适用于: IHC-Fr, IHC-P, WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Guinea pig, Hamster, Cow, Human, Pig, Saccharomyces cerevisiae, Monkey, Escherichia coli
  • 免疫原

    Human Hsp60 produced through recombinant DNA methods in E.coli

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab59458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 60 kDa.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • 疾病相关Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • 序列相似性Belongs to the chaperonin (HSP60) family.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all

Anti-Hsp60 antibody [LK-2] 图像

  • Lanes 1 - 3 : Anti-Hsp60 antibody [LK-2] (ab59458) at 1/4000 dilution
    Lane 4 :

    Lane 1 : Saccharomyces cerevisiae whole cell lysate at 6900000 cells
    Lane 2 : Saccharomyces cerevisiae whole cell lysate at 6900000 cells
    Lane 3 : Saccharomyces cerevisiae whole cell lysate at 6900000 cells
    Lane 4 : Ladder

    Secondary
    Lanes 1 - 2 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/5000 dilution
    Lane 3 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/2000 dilution


    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa


    Exposure time : 25 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-Hsp60 antibody [LK-2] (ab59458) at 1/1000 dilution + HeLa cell lysate

    Predicted band size : 60 kDa
  • ab59464 at 100,000 dilution staining Hsp60 alpha in human colon tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ system was used for staining. A HRP-conjugated anti mouse secondary was used at 1/10 dilution.

Anti-Hsp60 antibody [LK-2] (ab59458)参考文献

This product has been referenced in:
  • Bivi N  et al. Transcriptome and proteome analysis of osteocytes treated with nitrogen-containing bisphosphonates. J Proteome Res 8:1131-42 (2009). Read more (PubMed: 19226166) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 6.9e+006 cells
Gel Running Conditions Reduced Denaturing (4-12 % Bis-Tris, MOPS running buffer, semi-dry transfer to nitrocellulose)
Sample Saccharomyces cerevisiae Cell lysate - whole cell (W303alpha)
Specification W303alpha
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 25 2014

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