重组
RabMAb

Anti-Hsp60抗体[EP1006Y] - Loading Control (ab45134)

概述

  • 产品名称
    Anti-Hsp60抗体[EP1006Y] - Loading Control
    参阅全部 Hsp60 一抗
  • 描述
    兔单克隆抗体[EP1006Y] to Hsp60 - Loading Control
  • 经测试应用
    适用于: IHC-P, WBmore details
    不适用于: Flow Cyt,ICC/IF or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Hsp60 aa 400-500 (internal sequence).

  • 阳性对照
    • T47D cell lysate, human breast carcinoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab45134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
  • 应用说明
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • 靶标

    • 功能
      Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
    • 疾病相关
      Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
      Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
    • 序列相似性
      Belongs to the chaperonin (HSP60) family.
    • 细胞定位
      Mitochondrion matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 60 kDa chaperonin antibody
      • 60 kDa heat shock protein, mitochondrial antibody
      • CH60_HUMAN antibody
      • Chaperonin 60 antibody
      • Chaperonin, 60-KD antibody
      • CPN60 antibody
      • fa04a05 antibody
      • GROEL antibody
      • heat shock 60kDa protein 1 (chaperonin) antibody
      • Heat shock protein 1 (chaperonin) antibody
      • Heat shock protein 60 antibody
      • Heat shock protein 65 antibody
      • heat shock protein family D (Hsp60) member 1 antibody
      • HLD4 antibody
      • Hsp 60 antibody
      • HSP 65 antibody
      • HSP-60 antibody
      • HSP60 antibody
      • HSP65 antibody
      • HSPD1 antibody
      • HuCHA60 antibody
      • Mitochondrial matrix protein P1 antibody
      • P60 lymphocyte protein antibody
      • short heat shock protein 60 Hsp60s1 antibody
      • SPG13 antibody
      see all

    图片

    • All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/20000 dilution

      Lane 1 : T47-D (Human mammary gland ductal carcinoma) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma) whole cell lysates
      Lane 3 : 293T (Human embryonic kidney) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size : 60 kDa

      Blocking/dilution buffer: 5% NFDM /TBST

    • Immunohistochemistry analysis of paraffin-embedded Human stomach labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the glandular epithelium of the Human stomach shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

      Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

    • All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/50000 dilution

      Lane 1 : Human fetal brain lysates
      Lane 2 : Human fetal heart lysates
      Lane 3 : Human fetal kidney lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 60 kDa

      Blocking/dilution buffer: 5% NFDM /TBST

    • Immunohistochemistry analysis of paraffin-embedded Human transitional carcinoma of bladder labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on tumor cells of the Human bladder transitional cell carcinoma shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

      Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

    • Immunohistochemistry analysis of paraffin-embedded Mouse kidney labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the epithelium of Mouse kidney tubules shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

      Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

    • All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/50000 dilution

      Lane 1 : Mouse brain lysates
      Lane 2 : Mouse heart lysates
      Lane 3 : Rat brain lysates
      Lane 4 : Rat heart lysates
      Lane 5 : Raw264.7 (Mouse abelson murine leukemia virus-induced tumor) whole cell lysates
      Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
      Lane 7 : NIH/3T3 (Mouse embryo) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size : 60 kDa
    • Immunohistochemistry analysis of paraffin-embedded Rat liver labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the hepatocytes of the Rat liver shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

      Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

    文献

    This product has been referenced in:
    • Li Y  et al. Proteome Response of Chicken Embryo Fibroblast Cells to Recombinant H5N1 Avian Influenza Viruses with Different Neuraminidase Stalk Lengths. Sci Rep 7:40698 (2017). WB ; Chicken . Read more (PubMed: 28079188) »
    • Egger ME  et al. Inhibition of autophagy with chloroquine is effective in melanoma. J Surg Res 184:274-81 (2013). Read more (PubMed: 23706562) »

    See all 2 Publications for this product

    客户评价及客户问答

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human fibroblast cell and mitochondria fraction)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    100 µg
    Specification
    Human fibroblast cell and mitochondria fraction
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 09 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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