概述

  • 产品名称Anti-Hsp60抗体
    参阅全部 Hsp60 一抗
  • 描述
    小鼠多克隆抗体to Hsp60
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein corresponding to amino acids 1-573 of Human Hsp60 (NP_002147.2).

  • 阳性对照
    • Human kidney lysate, HepG2 lysate, Hsp60 transfected 293T cell lysate, HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab89609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • 疾病相关Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • 序列相似性Belongs to the chaperonin (HSP60) family.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all

Anti-Hsp60 antibody 图像

  • Anti-Hsp60 antibody (ab89609) at 1 µg/ml + Human kidney lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Anti-Hsp60 antibody (ab89609) at 1 µg/ml + HepG2 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
  • All lanes : Anti-Hsp60 antibody (ab89609) at 1 µg/ml

    Lane 1 : Hsp60 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ab89609 at 10 µg/ml staining Hsp60 in HeLa cells by Immunofluorescence.

Anti-Hsp60 antibody (ab89609)参考文献

ab89609 has not yet been referenced specifically in any publications.

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