概述

  • 产品名称Anti-Hsp60抗体
    参阅全部 Hsp60 一抗
  • 描述
    山羊多克隆抗体to Hsp60
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Dog, Pig, Xenopus laevis, Drosophila melanogaster, Fish, Monkey
  • 免疫原

    Recombinant protein (Human)

  • 阳性对照
    • WB: HeLa, NIH 3T3 or RK-13 cell lysate. IHC-P: adrenal, spleen or uterus tissue.

性能

应用

Our Abpromise guarantee covers the use of ab109873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 61 kDa.
IP 1/100.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • 疾病相关Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • 序列相似性Belongs to the chaperonin (HSP60) family.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all

Anti-Hsp60 antibody 图像

  • All lanes : Anti-Hsp60 antibody (ab109873) at 1/1000 dilution

    Lane 1 : NSP-540-Hsp60 active recombinant protein
    Lane 2 : SSP-610-GroEL active recombinant protein
    Lane 3 : NSP-581-Hsp65 active recombinant protein
    Lane 4 : HeLa cell lysate
    Lane 5 : NIH 3T3 cell lysate
    Lane 6 : RK-13 cell lysate


    Predicted band size : 61 kDa
  • ab109873 at 10 µg/ml staining Hsp60 in formalin-fixed, paraffin-embedded Human adrenal tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated anti-Goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab109873 at 10 µg/ml staining Hsp60 in formalin-fixed, paraffin-embedded Human spleen tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated anti-Goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab109873 at 10 µg/ml staining Hsp60 in formalin-fixed, paraffin-embedded Human uterus tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated anti-Goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Anti-Hsp60 antibody (ab109873)参考文献

This product has been referenced in:
  • Liu K  et al. Phosphorylated AKT inhibits the apoptosis induced by DRAM-mediated mitophagy in hepatocellular carcinoma by preventing the translocation of DRAM to mitochondria. Cell Death Dis 5:e1078 (2014). ICC/IF ; Human . Read more (PubMed: 24556693) »

See 1 Publication for this product

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