Anti-Hsp60抗体[1D11BD8] - Mitochondrial Marker (ab110312)

概述

  • 产品名称Anti-Hsp60抗体[1D11BD8] - Mitochondrial Marker
    参阅全部 Hsp60 一抗
  • 描述
    小鼠单克隆抗体[1D11BD8] to Hsp60 - Mitochondrial Marker
  • 经测试应用适用于: IHC-P, WB, ICC/IF, In-Cell ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Hela cell lysate

  • 阳性对照
    • Human heart tissue HepG2 Fibroblast cells HeLa cells Human normal colon FFPE tissue.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 61 kDa.
ICC/IF Use a concentration of 1 µg/ml.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • 疾病相关Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • 序列相似性Belongs to the chaperonin (HSP60) family.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all

Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker 图像

  • Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312) at 5 µg/ml + HepG2 whole cells at 10 µg

    Predicted band size : 61 kDa
  • ab110312 staining Hsp60 in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100 in BSA + 0.02% Tween20) for 1 hour at 16°C. A DyLight® 550-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry image of stained fibroblast cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110312 antibody (1 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent.
  • HeLa cells were stained with 1 µg/mL ab110312 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • IHC image of Hsp60 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110312, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312)参考文献

This product has been referenced in:
  • Magnoni R  et al. The Hsp60 folding machinery is crucial for manganese superoxide dismutase folding and function. Free Radic Res 48:168-79 (2014). Read more (PubMed: 24151936) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample Human Cell (MDA MB 231 cells)
Specification MDA MB 231 cells
Permeabilization Yes - 1% Triton
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 19 2015

Thank you for your enquiry and your interest in our products.
As far as we understand the epitipe has not been mapped yet so unfortunately I can't provide this information for you. ab110312 has been tested in several applications (IHC-P, WB, ICC/I...

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I'm happy to help! Please feel free to contact me if you need anything further.

There are lots of enzyme functions going on in the mitochondrial matrix. So lots of different options for WB in the matrix:


MCAD – human and rodent http://www.mitosciences.com/mcad.html

PDH E1a – human and rodent http://www....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"