RabMAb

Anti-Hsp47抗体[EPR4217] (ab109117)

概述

  • 产品名称Anti-Hsp47抗体[EPR4217]
    参阅全部 Hsp47 一抗
  • 描述
    兔单克隆抗体[EPR4217] to Hsp47
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • 阳性对照
    • HeLa, A431 and NIH/3T3 cell lysates; Human breast tissue; HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

     

    Alternative versions available:

    Anti-Hsp47 antibody (Alexa Fluor® 488) [EPR4217] (ab192841)
    Anti-Hsp47 antibody (Alexa Fluor® 647) [EPR4217] (ab192844)
    Anti-Hsp47 antibody (HRP) [EPR4217] (ab193242)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号EPR4217
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.

For unpurified use at 1/100.

IHC-P 1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See protocols http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol.

For unpurified use at 1/100 - 1/250.

靶标

  • 功能Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • 疾病相关Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass.
  • 序列相似性Belongs to the serpin family.
  • 细胞定位Endoplasmic reticulum lumen.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 47 kDa heat shock protein antibody
    • 47 kDa heat shock protein precursor antibody
    • Arsenic transactivated protein 3 antibody
    • Arsenic-transactivated protein 3 antibody
    • AsTP 3 antibody
    • AsTP3 antibody
    • CBP 1 antibody
    • CBP 2 antibody
    • CBP1 antibody
    • CBP2 antibody
    • Cell proliferation-inducing gene 14 protein antibody
    • Collagen binding protein 1 antibody
    • Collagen binding protein 2 antibody
    • Collagen binding protein antibody
    • Collagen-binding protein 2 antibody
    • Collagen-binding protein antibody
    • Colligen antibody
    • Colligin 1 antibody
    • Colligin 2 antibody
    • Colligin antibody
    • colligin-1 antibody
    • colligin-2 antibody
    • gp46 antibody
    • Heat shock protein 47 antibody
    • Heat-shock protein 47 antibody
    • HGNC 1547 antibody
    • Hsp 47 antibody
    • J6 antibody
    • OI10 antibody
    • PIG 14 antibody
    • PIG14 antibody
    • PPROM antibody
    • Proliferation inducing gene 14 antibody
    • Proliferation inducing gene 14 protein antibody
    • RA A47 antibody
    • RA-A47 antibody
    • Rheumatoid arthritis antigen A 47 antibody
    • rheumatoid arthritis antigen A-47 antibody
    • Rheumatoid arthritis related antigen RA A47 antibody
    • Rheumatoid arthritis-related antigen RA-A47 antibody
    • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody
    • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2) antibody
    • Serine or cysteine proteinase inhibitor clade H member 1 antibody
    • Serine or cysteine proteinase inhibitor clade H member 2 antibody
    • SERPH_HUMAN antibody
    • Serpin H1 antibody
    • Serpin peptidase inhibitor clade H member 1 antibody
    • serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody
    • Serpin peptidase inhibitor, clade H, member 1 antibody
    • SERPINH1 antibody
    • SERPINH2 antibody
    see all

Anti-Hsp47 antibody [EPR4217] 图像

  • ab109117 staining Hsp47 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.

  • All lanes : Anti-Hsp47 antibody [EPR4217] (ab109117) at 1/2000 dilution

    Lane 1 : A431 Cell Lysate
    Lane 2 : NIH/3T3 Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size : 46 kDa
  • All lanes : Anti-Hsp47 antibody [EPR4217] (ab109117) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 46 kDa
  • ab109117, unpurified, at 1/100 dilution, staining Hsp47 in Human breast tissue by Immunohistochemistry.

Anti-Hsp47 antibody [EPR4217] (ab109117)参考文献

This product has been referenced in:
  • Mahmud H  et al. Cardiac function and architecture are maintained in a model of cardiorestricted overexpression of the prorenin-renin receptor. PLoS One 9:e89929 (2014). WB ; Mouse . Read more (PubMed: 24587131) »
  • Wu ZB  et al. CTL responses to HSP47 associated with the prolonged survival of patients with glioblastomas. Neurology 82:1261-5 (2014). Human . Read more (PubMed: 24623841) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Mar 15 2016

Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Mar 15 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung tissue: fibroblasts)
Antigen retrieval step None
Permeabilization No
Specification Lung tissue: fibroblasts
Blocking step Zytomed Systems HRP-Polymer Kit Blocking Reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative HOPE-Fixation
Username

Abcam user community

Verified customer

提交于 Apr 13 2015

The concentration for lot YI081905CS is 2.0130 mg/ml.

Thank you for your enquiry.

I can confirm that ab109117anti-HSP47 antibody [EPR4217] is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (chondrocyte cell line ATDC5)
Loading amount 30 µg
Specification chondrocyte cell line ATDC5
Gel Running Conditions Reduced Denaturing (4-20% Bis-Tris)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
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Verified customer

提交于 Jun 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (hVSMCs)
Loading amount 5 µg
Specification hVSMCs
Gel Running Conditions Reduced Denaturing (10 %)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

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Verified customer

提交于 Aug 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"