Anti-Hsp47抗体[134CT7.1.8] (ab86750)


  • 产品名称Anti-Hsp47抗体[134CT7.1.8]
    参阅全部 Hsp47 一抗
  • 描述
    小鼠单克隆抗体[134CT7.1.8] to Hsp47
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat Hsp47.

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: NIH3T3; Human Placenta


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号134CT7.1.8
  • 同种型IgG1
  • 研究领域


Our Abpromise guarantee covers the use of ab86750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 功能Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • 疾病相关Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass.
  • 序列相似性Belongs to the serpin family.
  • 细胞定位Endoplasmic reticulum lumen.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 47 kDa heat shock protein antibody
    • 47 kDa heat shock protein precursor antibody
    • Arsenic transactivated protein 3 antibody
    • Arsenic-transactivated protein 3 antibody
    • AsTP 3 antibody
    • AsTP3 antibody
    • CBP 1 antibody
    • CBP 2 antibody
    • CBP1 antibody
    • CBP2 antibody
    • Cell proliferation-inducing gene 14 protein antibody
    • Collagen binding protein 1 antibody
    • Collagen binding protein 2 antibody
    • Collagen binding protein antibody
    • Collagen-binding protein 2 antibody
    • Collagen-binding protein antibody
    • Colligen antibody
    • Colligin 1 antibody
    • Colligin 2 antibody
    • Colligin antibody
    • colligin-1 antibody
    • colligin-2 antibody
    • gp46 antibody
    • Heat shock protein 47 antibody
    • Heat-shock protein 47 antibody
    • HGNC 1547 antibody
    • Hsp 47 antibody
    • J6 antibody
    • OI10 antibody
    • PIG 14 antibody
    • PIG14 antibody
    • PPROM antibody
    • Proliferation inducing gene 14 antibody
    • Proliferation inducing gene 14 protein antibody
    • RA A47 antibody
    • RA-A47 antibody
    • Rheumatoid arthritis antigen A 47 antibody
    • rheumatoid arthritis antigen A-47 antibody
    • Rheumatoid arthritis related antigen RA A47 antibody
    • Rheumatoid arthritis-related antigen RA-A47 antibody
    • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody
    • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2) antibody
    • Serine or cysteine proteinase inhibitor clade H member 1 antibody
    • Serine or cysteine proteinase inhibitor clade H member 2 antibody
    • SERPH_HUMAN antibody
    • Serpin H1 antibody
    • Serpin peptidase inhibitor clade H member 1 antibody
    • serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody
    • Serpin peptidase inhibitor, clade H, member 1 antibody
    • SERPINH1 antibody
    • SERPINH2 antibody
    see all

Anti-Hsp47 antibody [134CT7.1.8] 图像

  • All lanes : Anti-Hsp47 antibody [134CT7.1.8] (ab86750) at 5 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa

    Exposure time : 4 minutes
  • Overlay histogram showing HL60 cells stained with ab86750 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86750, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Hsp47 antibody [134CT7.1.8] (ab86750)参考文献

ab86750 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Skin fibroblasts)
Specification Skin fibroblasts
Fixative Formaldehyde
Permeabilization Yes - saponin

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提交于 Apr 02 2012

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