概述

  • 产品名称Anti-Hsp40抗体
    参阅全部 Hsp40 一抗
  • 描述
    兔多克隆抗体to Hsp40
  • 特异性This antibody detects a ~40 kDa protein, corresponding to the apparent molecular mass of Hsp40 on SDS-PAGE immunoblots. It does not detect DnaJ in E.coli lysates.
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Fish, Monkey
    不与反应: Escherichia coli
  • 免疫原

    Recombinant human Hsp40

  • 阳性对照
    • HeLa heat shocked cell lysate and recombinant human Hsp40 Protein

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • ��种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
IP 1/100.
ICC/IF 1/100 - 1/400.

靶标

Anti-Hsp40 antibody 图像

  • All lanes : Anti-Hsp40 antibody (ab69402) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Hsp40 recombinant protein
    Lane 3 : DnaJ recombinant protein
    Lane 4 : Cell lysates prepared from heat shocked hela cells
    Lane 5 : Cell lysates prepared from heat shocked 3T3 cells
    Lane 6 : Cell lysates prepared from PC-12 cells


    Predicted band size : 38 kDa
  • ab69402 staining Hsp40 in the A549 human lung cancer cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% BSA for 20 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 20°C. A CF568-conjugated Donkey anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody.

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Anti-Hsp40 antibody (ab69402)参考文献

This product has been referenced in:
  • Weber TA  et al. APOOL is a cardiolipin-binding constituent of the Mitofilin/MINOS protein complex determining cristae morphology in mammalian mitochondria. PLoS One 8:e63683 (2013). WB ; Human . Read more (PubMed: 23704930) »
  • Maffei S  et al. Beneficial effect of directional freezing on in vitro viability of cryopreserved sheep whole ovaries and ovarian cortical slices. Hum Reprod N/A:N/A (2013). IHC-Fr ; Sheep . Read more (PubMed: 24135077) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (A549 Lung cancer cell)
Specification A549 Lung cancer cell
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
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Dr. Dimitra Kalamida

Verified customer

提交于 Jun 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"