概述

  • 产品名称Anti-Hsp40抗体
    参阅全部 Hsp40 一抗
  • 描述
    兔多克隆抗体to Hsp40
  • 特异性ab53108 detects endogenous levels of total Hsp40 protein.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A synthetic peptide derived from human Hsp40.

  • 阳性对照
    • Human lung carcinoma tissue and COLO205 cell extracts.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/300 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 38 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
  • 序列相似性Contains 1 J domain.
  • 细胞定位Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DnaJ (Hsp40) homolog subfmaily B member 1 antibody
    • DNAJ 1 antibody
    • DNAJ B1 antibody
    • DnaJ homolog subfamily B member 1 antibody
    • DnaJ protein homolog 1 antibody
    • DNAJ1 antibody
    • DNAJB 1 antibody
    • Dnajb1 antibody
    • DNAJB1 protein antibody
    • DNJB1_HUMAN antibody
    • HDJ 1 antibody
    • HDJ-1 antibody
    • HDJ1 antibody
    • Heat shock 40 kDa protein 1 antibody
    • Heat shock 40kD protein 1 antibody
    • Heat shock protein 40 antibody
    • Hsp 40 antibody
    • HSP40 antibody
    • HSPF 1 antibody
    • HSPF1 antibody
    • Human DnaJ protein 1 antibody
    • Radial spoke 16 homolog B antibody
    • RSPH16B antibody
    • Sis1 antibody
    see all

Anti-Hsp40 antibody 图像

  • All lanes : Anti-Hsp40 antibody (ab53108) at 1/300 dilution

    Lane 1 : COLO205 cell extract
    Lane 2 : COLO205 cell extract with immunising peptide


    Predicted band size : 38 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
  • ab53108 at 1/50 dilution staining Hsp40 in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

Anti-Hsp40 antibody (ab53108)参考文献

ab53108 has not yet been referenced specifically in any publications.

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