Anti-Hsp27 (phospho S82)抗体(ab90537)

概述

  • 产品名称Anti-Hsp27 (phospho S82)抗体
    参阅全部 Hsp27 一抗
  • 描述
    兔多克隆抗体to Hsp27 (phospho S82)
  • 经测试应用适用于: WB, IP, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Chicken, Guinea pig, Cow, Dog, Human
  • 免疫原

    Synthetic phosphopeptide from a region around serine 82 (Human) conjugated to KLH

  • 阳性对照
    • Heat shocked HeLa cells; phosphorylated Hsp27 protein. This antibody gave a positive result in IHC in the following FFPE tissue: Human skeletal muscle.

性能

应用

Our Abpromise guarantee covers the use of ab90537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 23 kDa.
IP 1/100.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Involved in stress resistance and actin organization.
  • 组织特异性Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
  • 疾病相关Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
    Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
  • 序列相似性Belongs to the small heat shock protein (HSP20) family.
  • 翻译后修饰Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heat shock 27kDa protein antibody
    • 28 kDa heat shock protein antibody
    • CMT2F antibody
    • DKFZp586P1322 antibody
    • epididymis secretory protein Li 102 antibody
    • Estrogen regulated 24 kDa protein antibody
    • Estrogen-regulated 24 kDa protein antibody
    • Heat shock 25kDa protein 1 antibody
    • Heat shock 27 kDa protein antibody
    • Heat shock 27kD protein 1 antibody
    • Heat shock 27kDa protein 1 antibody
    • Heat shock 28kDa protein 1 antibody
    • Heat Shock Protein 27 antibody
    • Heat shock protein beta 1 antibody
    • Heat shock protein beta-1 antibody
    • heat shock protein family B (small) member 1 antibody
    • HEL-S-102 antibody
    • HMN2B antibody
    • HS.76067 antibody
    • Hsp 25 antibody
    • HSP 27 antibody
    • Hsp 28 antibody
    • Hsp B1 antibody
    • Hsp25 antibody
    • HSP27 antibody
    • Hsp28 antibody
    • HspB1 antibody
    • HSPB1_HUMAN antibody
    • SRP27 antibody
    • Stress responsive protein 27 antibody
    • Stress-responsive protein 27 antibody
    see all

Anti-Hsp27 (phospho S82) antibody 图像

  • IHC image of Hsp27 (phospho S82) staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90537, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab90537 staining Hsp27 in HeLa cells by Confocal Immunofluorescence (green). Nuclei are stained in blue pseudocolor using DRAQ5.
  • All lanes : Anti-Hsp27 (phospho S82) antibody (ab90537) at 1/2000 dilution

    Lane 1 : phosphorylated Hsp27 protein
    Lane 2 : Non-phosphorylated Hsp27 protein


    Predicted band size : 23 kDa

Anti-Hsp27 (phospho S82) antibody (ab90537)参考文献

ab90537 has not yet been referenced specifically in any publications.

Product Wall

According to our records, ab90537 was proving difficult to use inWB and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand th...

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Please send image as we discussed.

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please a...

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Thank you for contacting Abcam.

I am sorry about the issues you have been having with a few of our antibodies. These antibodies are covered under our Abpromise for six months and are guaranteed to work in western blot on rat samples .
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Thank you for contacting us.

As discussed any anti phopho HsP27 specific antibody can be used.

Please find attached the link to publication where author have used podocyte and anti Hsp27 p83 ab.

http://ajprenal.physiology...

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