Anti-Hsp27 (phospho S15)抗体[EP2293Y] (ab76313)
- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
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产品名称Anti-Hsp27 (phospho S15)抗体[EP2293Y]
参阅全部 Hsp27 一抗 -
描述兔单克隆抗体[EP2293Y] to Hsp27 (phospho S15)
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特异性ab76313 will detect Hsp27 phosphorylation on Serine 15.
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经测试应用适用于: WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性与反应: Human
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免疫原
A synthetic phospho-peptide corresponding to residues surrounding Serine 15 of human Hsp27.
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阳性对照
- HeLa cells, HeLa cell lysate heat treated at 44°C.
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常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.13% BSA -
Concentration information loading... -
纯度Protein A purified
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克隆单克隆
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克隆编号EP2293Y
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同种型IgG
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研究领域
应用
Our Abpromise guarantee covers the use of ab76313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/5000. Predicted molecular weight: 23 kDa. | |
| ICC/IF | 1/100 - 1/250. |
靶标
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功能Involved in stress resistance and actin organization.
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组织特异性Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
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疾病相关Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs. -
序列相似性Belongs to the small heat shock protein (HSP20) family.
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翻译后修饰Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
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细胞定位Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
- Information by UniProt
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数据库链接
- Entrez Gene: 3315 Human
- Omim: 602195 Human
- SwissProt: P04792 Human
- Unigene: 520973 Human
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别名
- Heat shock 27kDa protein antibody
- 28 kDa heat shock protein antibody
- CMT2F antibody
see all
Anti-Hsp27 (phospho S15) antibody [EP2293Y] 图像
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![Western blot - Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313)](http://www.abcam.cn/ps/products/76/ab76313/Images/ab76313-258924-ab76313WB1.jpg)
Western blot - Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313)All lanes : Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313) at 1/2500 dilution
Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysates
Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size : 23 kDa
Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands. -
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313)](http://www.abcam.cn/ps/products/76/ab76313/Images/ab76313-258923-ab76313ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313)ab76313 staining Hsp27 (phospho S15) in HeLa (human cervix adenocarcinoma) cells with heat treatment at 44°C. by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/150. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab76313 (1/1000) and secondary antibody ab150077 (1/1000) respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)
Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313)参考文献
This product has been referenced in:
- Adam AP et al. Src Family Kinases Modulate the Loss of Endothelial Barrier Function in Response to TNF-a: Crosstalk with p38 Signaling. PLoS One 11:e0161975 (2016). Read more (PubMed: 27603666) »
- Tate CM et al. LY2228820 dimesylate, a selective inhibitor of p38 mitogen-activated protein kinase, reduces angiogenic endothelial cord formation in vitro and in vivo. J Biol Chem 288:6743-53 (2013). WB . Read more (PubMed: 23335506) »
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