Anti-Hsp27抗体[EP1724Y] (ab62339)


  • 产品名称Anti-Hsp27抗体[EP1724Y]
    参阅全部 Hsp27 一抗
  • 描述
    兔单克隆抗体[EP1724Y] to Hsp27
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human Hsp27

  • 阳性对照
    • HeLa cell lysate, human cervical carcinoma tissue, human breast carcinoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.




Our Abpromise guarantee covers the use of ab62339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Involved in stress resistance and actin organization.
    • 组织特异性Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
    • 疾病相关Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
      Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
    • 序列相似性Belongs to the small heat shock protein (HSP20) family.
    • 翻译后修饰Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
    • 细胞定位Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Heat shock 27kDa protein antibody
      • 28 kDa heat shock protein antibody
      • CMT2F antibody
      • DKFZp586P1322 antibody
      • epididymis secretory protein Li 102 antibody
      • Estrogen regulated 24 kDa protein antibody
      • Estrogen-regulated 24 kDa protein antibody
      • Heat shock 25kDa protein 1 antibody
      • Heat shock 27 kDa protein antibody
      • Heat shock 27kD protein 1 antibody
      • Heat shock 27kDa protein 1 antibody
      • Heat shock 28kDa protein 1 antibody
      • Heat Shock Protein 27 antibody
      • Heat shock protein beta 1 antibody
      • Heat shock protein beta-1 antibody
      • heat shock protein family B (small) member 1 antibody
      • HEL-S-102 antibody
      • HMN2B antibody
      • HS.76067 antibody
      • Hsp 25 antibody
      • HSP 27 antibody
      • Hsp 28 antibody
      • Hsp B1 antibody
      • Hsp25 antibody
      • HSP27 antibody
      • Hsp28 antibody
      • HspB1 antibody
      • HSPB1_HUMAN antibody
      • SRP27 antibody
      • Stress responsive protein 27 antibody
      • Stress-responsive protein 27 antibody
      see all

    Anti-Hsp27 antibody [EP1724Y] 图像

    • Anti-Hsp27 antibody [EP1724Y] (ab62339) at 1/500 dilution + HeLa cell lysate at 10 µg

      goat anti rabbit, HRP labeled, at 1/2000 dilution

      Predicted band size : 23 kDa
      Observed band size : 23 kDa
    • Immunohistochemical analysis of Hsp27 expression in paraffin embedded human cervical carcinoma (left) and human breast carcinoma (right) using 1/100 ab62339

    Anti-Hsp27 antibody [EP1724Y] (ab62339)参考文献

    ab62339 has not yet been referenced specifically in any publications.

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