概述

  • 产品名称Anti-Hsp27抗体[B317]
    参阅全部 Hsp27 一抗
  • 描述
    小鼠单克隆抗体[B317] to Hsp27
  • 特异性This antibody, also known as the estrogen regulated 24 kD protein and HSP 28, is one of the several heat shock proteins (HSP) produced by all organisms studied. The antigen is induced by elevated temperature as well as estrogen in hormone response. This protein has been studied in human estrogen response tissues such as cervix, endometrium and breast tissue. It may be useful in classification of various hormone sensitive tumors.
  • 经测试应用适用于: IHC-FoFr, IHC-Fr, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein corresponding to Human Hsp27. Partially purified HSP27 derived from MCF-7 cytosol.

  • 阳性对照
    • Breast Carcinoma.
  • 常规说明Prolonged fixation in buffered formalin can destroy the epitope.

性能

应用

Our Abpromise guarantee covers the use of ab8600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/50 - 1/100.
IHC-Fr 1/50 - 1/100.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/300.

靶标

  • 功能Involved in stress resistance and actin organization.
  • 组织特异性Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
  • 疾病相关Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
    Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
  • 序列相似性Belongs to the small heat shock protein (HSP20) family.
  • 翻译后修饰Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heat shock 27kDa protein antibody
    • 28 kDa heat shock protein antibody
    • CMT2F antibody
    • DKFZp586P1322 antibody
    • epididymis secretory protein Li 102 antibody
    • Estrogen regulated 24 kDa protein antibody
    • Estrogen-regulated 24 kDa protein antibody
    • Heat shock 25kDa protein 1 antibody
    • Heat shock 27 kDa protein antibody
    • Heat shock 27kD protein 1 antibody
    • Heat shock 27kDa protein 1 antibody
    • Heat shock 28kDa protein 1 antibody
    • Heat Shock Protein 27 antibody
    • Heat shock protein beta 1 antibody
    • Heat shock protein beta-1 antibody
    • heat shock protein family B (small) member 1 antibody
    • HEL-S-102 antibody
    • HMN2B antibody
    • HS.76067 antibody
    • Hsp 25 antibody
    • HSP 27 antibody
    • Hsp 28 antibody
    • Hsp B1 antibody
    • Hsp25 antibody
    • HSP27 antibody
    • Hsp28 antibody
    • HspB1 antibody
    • HSPB1_HUMAN antibody
    • SRP27 antibody
    • Stress responsive protein 27 antibody
    • Stress-responsive protein 27 antibody
    see all

Anti-Hsp27 antibody [B317] 图像

  • All lanes : Anti-Hsp27 antibody [B317] (ab8600) at 1/300 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa (possible post-translational modification).
  • ab8600 staining Hsp27 in human normal skin tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation and heat mediated antigen retrieval in Citrate buffer pH 6.0. The primary antibody was diluted, 1/50 in PBT (PBS + 0.5% Tween-20 + 0.5% BSA) and incubated with sample for 20 hours at 21°C. An Alexa Fluor® 594 conjugated Donkey polyclonal to mouse IgG
    was used as secondary at 1/100 dilution, for 1 hour at 21°C. ab8600 show red staining of Hsp27 in image. Counterstain with DAPI was performed by adding DAPI in a final concentration of 0.5 µg/ml to the mounting medium.

    See Abreview

  • All lanes : Anti-Hsp27 antibody [B317] (ab8600) at 0.83 µg/ml

    Lane 1 : Human HaCaT Whole cell lysate
    Lane 2 : Human HaCaT Whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    An HRP-conjugated goat anti-mouse polyclonal at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

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Anti-Hsp27 antibody [B317] (ab8600)参考文献

This product has been referenced in:
  • Gawlowski T  et al. Heat shock protein 27 modification is increased in the human diabetic failing heart. Horm Metab Res 41:594-9 (2009). WB . Read more (PubMed: 19384818) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HaCaT)
Loading amount 30 µg
Specification HaCaT
Gel Running Conditions Reduced Denaturing (15%, 6V, 60 min.)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

提交于 Mar 05 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Normal Skin)
Specification Normal Skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat, pH 6.0
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Jan 27 2010

Thank you for your enquiry. Hsp27 is cytoplasmic in interphase cells, colocalizes with mitotic spindles in mitotic cells and translocates to the nucleus during heat shock. I would recommend that your customer uses a RIPA buffer extraction. I ho...

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Thank you for your enquiry. I am sorry to hear that your customer has been having difficulties with this antibody. I have read your customer's questionaire and they are following a protocol that I would largely recommend. However, I would appre...

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We do not know for sure whether this anitbody recognises phosphorylated and non-phosphorylated forms of HSP27. It might be possible to find the answer in published work involving this antibody - there are five listed reference on the datasheet. We do ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"