概述

  • 产品名称
  • 描述
    山羊多克隆抗体to Hsp22
  • 经测试应用
    适用于: WB, ICCmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    NELPQDSQEVTCT

    , corresponding to C terminal amino acids 184-196 of Human Hsp22.

  • 阳性对照
    • Human Muscle lysate.
  • 常规说明
    Principal Names - H11; E2IG1; HSP22; HspB8; protein kinase H11; small stress protein-like protein HSP22 Official Gene Symbol - H11 (interim symbol) GenBank Accession Number – NP_055180 LocusLink ID - 26353 (human) Gene Ontology terms - protein serine/threonine kinase activity; heat shock protein activity; biological_process unknown; cellular_component unknown; transferase activity.

性能

应用

Our Abpromise guarantee covers the use of ab4149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20-25 kDa (predicted molecular weight: 22 kDa).Can be blocked with Human Hsp22 peptide (ab22992).

Approx 20-25 kDa band seen in Human Muscle lysate [Predicted MW of approx. 22 kDa according to NP_055180].

ICC Use at an assay dependent concentration.

靶标

  • 功能
    Displays temperature-dependent chaperone activity.
  • 组织特异性
    Predominantly expressed in skeletal muscle and heart.
  • 疾病相关
    Neuronopathy, distal hereditary motor, 2A
    Charcot-Marie-Tooth disease 2L
  • 序列相似性
    Belongs to the small heat shock protein (HSP20) family.
  • 细胞定位
    Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha crystallin C chain antibody
    • Alpha-crystallin C chain antibody
    • Charcot Marie Tooth disease axonal type 2L antibody
    • Charcot Marie Tooth disease spinal antibody
    • CMT2L antibody
    • CRYAC antibody
    • DHMN 2 antibody
    • DHMN2 antibody
    • E2 induced gene 1 protein antibody
    • E2-induced gene 1 protein antibody
    • E2IG1 antibody
    • H11 antibody
    • Heat shock 22kDa protein 8 antibody
    • Heat shock 27kDa protein 8 antibody
    • Heat shock protein 22 antibody
    • Heat shock protein beta 8 antibody
    • Heat shock protein beta-8 antibody
    • Hereditary motor neuropathy distal antibody
    • HMN 2 antibody
    • HMN2 antibody
    • HMN2A antibody
    • HSB8 antibody
    • HSPB 8 antibody
    • HspB8 antibody
    • HSPB8_HUMAN antibody
    • OTTHUMP00000239768 antibody
    • Protein kinase H11 antibody
    • Small stress protein like protein HSP22 antibody
    • Small stress protein-like protein HSP22 antibody
    • Spinal muscular atrophy distal adult autosomal dominant antibody
    see all

图片



  • Predicted band size : 22 kDa


    ab4149 staining (1µg/ml) of Human Muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

文献

This product has been referenced in:
  • Sarparanta J  et al. Mutations affecting the cytoplasmic functions of the co-chaperone DNAJB6 cause limb-girdle muscular dystrophy. Nat Genet 44:450-5 (2012). Read more (PubMed: 22366786) »
  • Laure L  et al. Cardiac H11 kinase/Hsp22 stimulates oxidative phosphorylation and modulates mitochondrial reactive oxygen species production: Involvement of a nitric oxide-dependent mechanism. Free Radic Biol Med 52:2168-2176 (2012). WB . Read more (PubMed: 22542467) »

See all 3 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Heart)
Permeabilization
Yes - Tween-20
Specification
Embryonic Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 22 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 21 2016

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Loading amount
60 µg
Specification
Hela
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Mr. Jörg Hoehfeld

Verified customer

提交于 Jun 27 2007

Thank you for taking the time to contact me regarding your experiences using this product, I am sorry to hear that you have been experiencing problems using this product in Western blotting. The working dilution (assuming a start conc. of 0.5mg/ml) ran...

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Thank you for submitting the completed Western Blot Questionnaire Form. We are very sorry to hear that you are having problem with this antibody. We would like to make the following comments/suggestions: 1. This antibody has been tested and char...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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