The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 (or 1 µl per 15 µg of heat shocked Arabidopsis thaliana lysate) with ECL. Goat anti rabbit IgG:HRP was used as the secondary
antibody. Predicted molecular weight: 18 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
HSP17.7 belongs to the class II family of small heat shock proteins which are induced when plant cells are stressed by increased temperature.
Small heat shock proteins appear to be involved in chaperone functions by protecting other proteins from irreversible denaturation. They are also produced late in the seed maturation process.