The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明WB: 1/5000 (or 0.2 µl/ml for 5 µg of Synechocystis PCC 6803 lysate) by ECL. Goat anti rabbit IgG:HRP was used as the secondary antibody. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Note: on a gradient gel this protein runs closer to 11 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性HSP16.6 belongs to the class I family of small heat shock proteins. They are induced once a cell is stressed by an increase in temperature. Synechocystis Hsp16.6 is involved in the development of thermo tolerance and thylakoid stability. Small heat shock proteins appear to be involved in chaperone functions by protecting other proteins from irreversible denaturation.
Small heat-shock protein HSP16.6 antibody
Anti-HSP16.6 antibody 图像
Western blot - HSP16.6 antibody (ab80184)
All lanes : Anti-HSP16.6 antibody (ab80184) at 1/5000 dilution
Lane 1 : Total protein lysates from wild type cyanobacteria Lane 2 : HSP16.6 knocked protein lysates from cyanobacteria
Lysates/proteins at 5 µg per lane.
Predicted band size : 17 kDa
Anti-HSP16.6 antibody (ab80184)参考文献
has not yet been referenced specifically in any publications.