概述

  • 产品名称Anti-Hsp105抗体[J1G12]
    参阅全部 Hsp105 一抗
  • 描述
    小鼠单克隆抗体[J1G12] to Hsp105
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human Hsp105 (1-858 aa) purified from E. coli

  • 阳性对照
    • HeLa cell lysate.
  • 常规说明


    ab74174, clone J1G12, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HSP105 protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Primary antibody说明ab74174, clone J1G12, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HSP105 protein.
  • 克隆单克隆
  • 克隆编号J1G12
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 97 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF 1/200.

靶标

  • 功能Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.
  • 组织特异性Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
  • 序列相似性Belongs to the heat shock protein 70 family.
  • 翻译后修饰Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antigen NY CO 25 antibody
    • Antigen NY-CO-25 antibody
    • DKFZp686M05240 antibody
    • Heat shock 105kD alpha antibody
    • Heat shock 105kD antibody
    • Heat shock 105kD beta antibody
    • Heat shock 105kDa protein 1 antibody
    • Heat shock 105kDa protein antibody
    • Heat shock 105kDa/110kDa protein 1 antibody
    • Heat shock 110 kDa protein antibody
    • Heat shock 110kDa protein antibody
    • Heat shock protein 105 kDa antibody
    • HS105_HUMAN antibody
    • HSP105 antibody
    • HSP105A antibody
    • HSP105B antibody
    • HSP110 antibody
    • HSPH 1 antibody
    • Hsph1 antibody
    • KIAA0201 antibody
    • NY CO 25 antibody
    see all

Anti-Hsp105 antibody [J1G12] 图像

  • Anti-Hsp105 antibody [J1G12] (ab74174) at 1/1000 dilution + HeLa cell lysate at 30 µg

    Secondary
    Goat anti mouse antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 97 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • ab74174 (1/200) staining Hsp105 in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

Anti-Hsp105 antibody [J1G12] (ab74174)参考文献

This product has been referenced in:
  • Zappasodi R  et al. Serological identification of HSP105 as a novel non-Hodgkin lymphoma therapeutic target. Blood 118:4421-30 (2011). WB ; Human . Read more (PubMed: 21860023) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 May 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"