产品名称 Anti-Hsp105抗体[J1G12] 参阅全部 Hsp105 一抗
小鼠单克隆抗体[J1G12] to Hsp105
WB, ELISA, ICC/IF more details
Recombinant full length human Hsp105 (1-858 aa) purified from E. coli
ab74174, clone J1G12, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HSP105 protein.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Primary antibody说明 ab74174, clone J1G12, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HSP105 protein.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 97 kDa.
Use at an assay dependent dilution.
功能 Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.
组织特异性 Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
序列相似性 Belongs to the heat shock protein 70 family.
翻译后修饰 Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
Information by UniProt
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Anti-Hsp105 antibody [J1G12] 图像
Western blot - Hsp105 antibody [J1G12] (ab74174)
Anti-Hsp105 antibody [J1G12] (ab74174) at 1/1000 dilution + HeLa cell lysate at 30 µg Secondary Goat anti mouse antibody conjugated to HRP Developed using the ECL technique Predicted band size : 97 kDa Observed band size : 110 kDa ( why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Hsp105 antibody [J1G12] (ab74174) This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab74174 (1/200) staining Hsp105 in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"