• 产品名称Anti-Hsp105抗体
    参阅全部 Hsp105 一抗
  • 描述
    兔多克隆抗体to Hsp105
  • 特异性ab53107 detects endogenous levels of total Hsp105 protein.
  • 经测试应用适用于: ICC/IF, WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    A synthetic peptide derived from human Hsp105.

  • 阳性对照
    • Human colon carcinoma tissue and HeLa cell extracts.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab53107 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab53107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/300 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.


  • 功能Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.
  • 组织特异性Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
  • 序列相似性Belongs to the heat shock protein 70 family.
  • 翻译后修饰Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antigen NY CO 25 antibody
    • Antigen NY-CO-25 antibody
    • DKFZp686M05240 antibody
    • Heat shock 105kD alpha antibody
    • Heat shock 105kD antibody
    • Heat shock 105kD beta antibody
    • Heat shock 105kDa protein 1 antibody
    • Heat shock 105kDa protein antibody
    • Heat shock 105kDa/110kDa protein 1 antibody
    • Heat shock 110 kDa protein antibody
    • Heat shock 110kDa protein antibody
    • Heat shock protein 105 kDa antibody
    • HS105_HUMAN antibody
    • HSP105 antibody
    • HSP105A antibody
    • HSP105B antibody
    • HSP110 antibody
    • HSPH 1 antibody
    • Hsph1 antibody
    • KIAA0201 antibody
    • NY CO 25 antibody
    see all

Anti-Hsp105 antibody 图像

  • All lanes : Anti-Hsp105 antibody (ab53107) at 1/300 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising peptide

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • ab53107 at 1/50 dilution staining Hsp105 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
  • ICC/IF image of ab53107 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53107, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

Anti-Hsp105 antibody (ab53107)参考文献

ab53107 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. The following reference reports HSP70 in the nuclei of cells at the onset of caspase activation: Nature. 2007 Jan 4;445(7123):102-5. Epub 2006 Dec 10. PMID: 17167422 and this reference describes the subcellular l...

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