概述

  • 产品名称
  • 描述
    兔多克隆抗体to Hsp104
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Hamster, Saccharomyces cerevisiae
    不与反应: Drosophila melanogaster, Plants, Escherichia coli
  • 免疫原

    Synthetic peptide corresponding to Saccharomyces cerevisiae Hsp104 aa 894-908 (C terminal).
    Sequence:

    DDDNEDSMEIDDDLD

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium azide
  • 纯度
    Whole antiserum
  • Primary antibody说明
    Heat Shock Proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP 70, HSP 90 and HSP 100. The HSP 100 proteins generally have amino acid sequences of about 900 residues and contain two nucleotide-binding sites. Within the HSP 100 family of proteins, yeast express an ~104 kDa form which is necessary to protect cells from various stress conditions such as heat, heavy metals and ethanol, though mutation studies have shown that the protein is not required for normal growth. Yeast HSP 104 has been shown to be a ClpB protein with significant sequence homology to E. coli ClpB, particularly in the two nucleotide-binding sites.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/5000. Detects a band of approximately 100-105 kDa. 1/100 for Mammals and 1/500 for yeast.
IHC-P 1/100 - 1/200.

靶标

  • 相关性
    Hsp104 is a molecular chaperone required for stress tolerance and for maintenance of [psi(+)] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 is vital to protect yeast cells against high temperature and high concentration of ethanol. It is not required for normal growth.
  • 细胞定位
    Cytoplasmic and Nuclear
  • 数据库链接
    • 别名
      • L0948 antibody
      • Heat shock protein 104 antibody
      • Hsp 104 antibody

    Anti-Hsp104 antibody (ab2924)参考文献

    This product has been referenced in:
    • Hamdan N  et al. ER stress causes widespread protein aggregation and prion formation. J Cell Biol 216:2295-2304 (2017). WB ; Saccharomyces cerevisiae . Read more (PubMed: 28630146) »
    • Park YN  et al. Hsp104 overexpression cures Saccharomyces cerevisiae [PSI+] by causing dissolution of the prion seeds. Eukaryot Cell 13:635-47 (2014). WB . Read more (PubMed: 24632242) »

    See all 5 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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