HsMyoD1 ChIP探针(ab83599)
概述
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产品名称
HsMyoD1 ChIP探针
参阅全部 MyoD1 dna, rna, cdna -
常规说明
Accession number: NM_002478.The HsMYOD1 primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. MYOD1 (Myoblast determination protein 1) is involved in muscle development. The primers and probe are located in exon 1. In most cell types MYOD1 is enriched with histone modifications associated with silencing (e.g. Histone H3 K27 di methylation, see image with ab24684). Whereas it shows low levels of histone modifications associated with active gene transcription such as Histone H3 K9 acetylation (see image with ab4441).
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经测试应用
适用于: Real Time PCRmore details
性能
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形式
Liquid -
存放说明
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: Water -
Concentration information loading...
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研究领域
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功能
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻译后修饰
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation. -
细胞定位
Nucleus. - Information by UniProt
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别名
- bHLHc1
- Class C basic helix-loop-helix protein 1
- MYF 3
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab83599于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Real Time PCR |
Use at an assay dependent concentration.
Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. The probe is labelled with FAM/TAMRA. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product. |
说明 |
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Real Time PCR
Use at an assay dependent concentration. Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. The probe is labelled with FAM/TAMRA. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product. |
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab83599 尚未被引用在任何文献中。