Hsgamma Actin promoter ChIP引物(Reverse) (ab83624)

概述

  • 产品名称Hsgamma Actin promoter ChIP引物(Reverse)
    参阅全部 gamma Actin promoter dna, rna, cdna
  • 常规说明


    Accession number: NG 011433.
    The HsACT1 primers can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. Gamma actin is a constitutively expressed gene. The primers are located around the core promoter of the ? actin gene. In most cell types the gamma actin promoter is enriched with histone modifications and core promoter binding factors (e.g. TBP, see image with ab51841) associated with active gene transcription. Together with the HsACT2 and HsACT3 primers these primers can be used to determine the abundance of a modification (e.g. Histone H3 K4 mono methylation, see image with ab8895) or a factor (e.g. RNA polymerase II, see image with ab26721) over the gamma actin locus.

  • 经测试应用适用于: Real Time PCRmore details

性能

  • 相关性Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates there are three main groups of actin isoforms, alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Defects in ACTG1 are the cause of non-syndromic sensorineural deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26).
  • 细胞定位Cytoplasmic
  • 别名
    • ACT
    • ACTG
    • Actin, cytoplasmic 2
    • Actin, gamma 1 propeptide
    • Cytoskeletal gamma actin
    • Deafness, autosomal dominant 20
    • Deafness, autosomal dominant 26
    • DFNA20
    • DFNA26
    see all

应用

Our Abpromise guarantee covers the use of ab83624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Real Time PCR Use at an assay dependent dilution. Suitable for the analysis of chromatin immunoprecipitated DNA using Sybr® green real time PCR. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.

Hsgamma Actin promoter ChIP primer (Reverse) (ab83624)参考文献

ab83624 has not yet been referenced specifically in any publications.

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