HsGAD67 ChIP引物(Forward) (ab83614)

概述

  • 产品名称
    HsGAD67 ChIP引物(Forward)
    参阅全部 GAD67 dna, rna, cdna
  • 常规说明

    Accession number: NM_000817.The HsGAD1 primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. GAD1 (Glutamic acid decarboxylase) is expressed in neurons but silenced in most other cell types. The primers and probe are located in exon 1, within 1 kb of the transcription start site. In most cell types GAD1 is enriched with histone modifications associated with silencing (e.g. Histone H3 K9 mono methylation, see image with ab9045). Whereas it shows low levels of histone modifications associated with active gene transcription such as Histone H3 K9 acetylation (see image with ab4441).

  • 经测试应用
    适用于: Real Time PCRmore details

性能

  • 形式
    Liquid
  • 存放说明
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Constituents: ddH20
  • Concentration information loading...
  • 研究领域
  • 功能
    Catalyzes the production of GABA.
  • 组织特异性
    Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
  • 疾病相关
    Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
  • 序列相似性
    Belongs to the group II decarboxylase family.
  • Information by UniProt
  • 别名
    • 67 kDa glutamic acid decarboxylase
    • CPSQ1
    • DCE1
    • DCE1_HUMAN
    • EC 4.1.1.15
    • FLJ45882
    • GAD
    • GAD 67
    • GAD-67
    • GAD1
    • Glutamate decarboxylase 1
    • Glutamate decarboxylase 1 (brain, 67kDa)
    • Glutamate decarboxylase 1 brain 67kD
    • Glutamate decarboxylase 1 brain 67kDa
    • Glutamate decarboxylase 67 kDa isoform
    • Glutamate decarboxylase, brain, 67-KD
    • OTTHUMP00000041055
    • SCP
    see all

应用

Our Abpromise guarantee covers the use of ab83614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Real Time PCR Use at an assay dependent concentration.

Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.

文献

ab83614 has not yet been referenced specifically in any publications.

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