Anti-HSF4抗体[EP1719Y] (ab53291)


  • 产品名称
    参阅全部 HSF4 一抗
  • 描述
    兔单克隆抗体[EP1719Y] to HSF4
  • 宿主
  • 经测试应用
    适用于: Flow Cyt, WB, ICC, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human HSF4 aa 250-350. The exact sequence is proprietary.

  • 阳性对照
    • HeLa cell lysate Human muscle
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents




Our Abpromise guarantee covers the use of ab53291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.
WB 1/5000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ICC 1/50 - 1/100.
IHC-P Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      DNA-binding protein that specifically binds heat shock promoter elements (HSE). Isoform HSF4A represses transcription while the isoform HSF4B activates transcription.
    • 组织特异性
      Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues.
    • 疾病相关
      Defects in HSF4 are the cause of cataract zonular HSF4-related (CZ-HSF4) [MIM:116800]. A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes.
      Defects in HSF4 are the cause of cataract Marner type (CAM) [MIM:116800]. A form of cataract with variable and progressive opacities. Affected individuals present with zonular cataract, although some have nuclear, anterior polar, or stellate cataract. Finger malformation is observed in some kindreds.
    • 序列相似性
      Belongs to the HSF family.
    • 翻译后修饰
      Phosphorylated mainly on serine residues. Phosphorylation on Ser-298 promotes sumoylation on Lys-293.
      Isoform HSF4B is constitutively sumoylated. Sumoylation represses the transcriptional activity and is promoted by phosphorylation on Ser-298. HSFA is not sumoylated.
    • 细胞定位
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cataract, Marner antibody
      • CTM antibody
      • Heat shock factor protein 4 antibody
      • Heat shock transcription factor 4 antibody
      • hHSF4 antibody
      • HSF 4 antibody
      • HSF4 antibody
      • HSF4_HUMAN antibody
      • HSTF 4 antibody
      see all


    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling HSF4 with unpurified ab53291 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Immunohistochemical analysis of human muscle with ab53291 at 1/50 - 1/100 dilution
    • Anti-HSF4 antibody [EP1719Y] (ab53291) at 1/10000 dilution + HeLa cell lysate at 10 µg

      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 53 kDa
      Observed band size: 53 kDa
      Additional bands at: 120 kDa, 40 kDa. We are unsure as to the identity of these extra bands.


    ab53291 has not yet been referenced specifically in any publications.


    Thank you for contacting Abcam regarding ab53291.

    I have confirmed with the lab that this antibody reacts with the HSF4A isoform only, and the immunogen does not share any significant sequence similarity with HSF4B.

    I ho...

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