Anti-HSF1 (phospho S326)抗体(ab115702)


  • 产品名称
    Anti-HSF1 (phospho S326)抗体
    参阅全部 HSF1 一抗
  • 描述
    兔多克隆抗体to HSF1 (phospho S326)
  • 宿主
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from Human HSF1 phosphorylated at Ser 326.

  • 阳性对照
    • HeLa cell lysate; HeLa cell lysate Heat-Shocked;



Our Abpromise guarantee covers the use of ab115702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 57 kDa). (ECL).


  • 功能
    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • 序列相似性
    Belongs to the HSF family.
  • 结构域
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 翻译后修饰
    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
  • 细胞定位
    Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heat shock factor 1 antibody
    • Heat shock factor protein 1 antibody
    • Heat shock transcription factor 1 antibody
    • HSF 1 antibody
    • hsf1 antibody
    • HSF1_HUMAN antibody
    • HSTF 1 antibody
    • HSTF1 antibody
    see all


  • All lanes : Anti-HSF1 (phospho S326) antibody (ab115702) at 1/1000 dilution

    Lane 1 : MW marker
    Lane 2 : HeLa cell lysate
    Lane 3 : HeLa cell lysate Heat-Shocked (No recovery time)

    Predicted band size: 57 kDa

    ab115702 detects a band of 85-95kDa by Western blot depending on hyperphosphorylation of the native protein.
  • All lanes : Anti-HSF1 (phospho S326) antibody (ab115702) at 1/1000 dilution

    Lane 1 : MW markers
    Lane 2 : Recombinant HSF1
    Lane 3 : Recombinant HSF1 (phospho)

    Predicted band size: 57 kDa



This product has been referenced in:
  • Lee JH  et al. Mitochondrial Respiratory Dysfunction Induces Claudin-1 Expression via Reactive Oxygen Species-mediated Heat Shock Factor 1 Activation, Leading to Hepatoma Cell Invasiveness. J Biol Chem 290:21421-31 (2015). Read more (PubMed: 26157141) »

See 1 Publication for this product


J’ai contacté le laboratoire pour savoir si l’un de nos anti- HSF1 (phospho S326) anticorps fonctionnerait chez la vache. Malheureusement, nous n’avons que trois anticorps : ab76076, ab1050 et ab115702 au catalogue et ils ont une homologie de 64%, 77% ...

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Merci de nous avoir contactés.
Nous sommes heureux d’avoir la possibilité de vous proposer un service par Epitomics, une société Abcam qui peut vous préparer des anticorps spécifiques.
Pour plus d'informations, visitez: http://www.epitomics.c...

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