Anti-HSF1 (phospho S326)抗体(ab115702)
Key features and details
- Rabbit polyclonal to HSF1 (phospho S326)
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-HSF1 (phospho S326)抗体
参阅全部 HSF1 一抗 -
描述
兔多克隆抗体to HSF1 (phospho S326) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human HSF1 (phospho S326).
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阳性对照
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115702于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 57 kDa). (ECL).
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说明 |
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WB
1/1000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 57 kDa). (ECL). |
靶标
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功能
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. -
序列相似性
Belongs to the HSF family. -
结构域
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
翻译后修饰
Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules. - Information by UniProt
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数据库链接
- Entrez Gene: 3297 Human
- Omim: 140580 Human
- SwissProt: Q00613 Human
- Unigene: 530227 Human
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别名
- Heat shock factor 1 antibody
- Heat shock factor protein 1 antibody
- Heat shock transcription factor 1 antibody
see all
图片
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All lanes : Anti-HSF1 (phospho S326) antibody (ab115702) at 1/1000 dilution
Lane 1 : MW marker
Lane 2 : HeLa cell lysate
Lane 3 : HeLa cell lysate Heat-Shocked (No recovery time)
Predicted band size: 57 kDa
ab115702 detects a band of 85-95kDa by Western blot depending on hyperphosphorylation of the native protein. -
All lanes : Anti-HSF1 (phospho S326) antibody (ab115702) at 1/1000 dilution
Lane 1 : MW markers
Lane 2 : Recombinant HSF1
Lane 3 : Recombinant HSF1 (phospho)
Predicted band size: 57 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab115702 被引用在 8 文献中.
- Fujimoto M et al. HSF1 phosphorylation establishes an active chromatin state via the TRRAP-TIP60 complex and promotes tumorigenesis. Nat Commun 13:4355 (2022). PubMed: 35906200
- Moreno R et al. The stress-responsive kinase DYRK2 activates heat shock factor 1 promoting resistance to proteotoxic stress. Cell Death Differ 28:1563-1578 (2021). PubMed: 33268814
- Trivedi R et al. Augmentation of the heat shock axis during exceptional longevity in Ames dwarf mice. Geroscience 43:1921-1934 (2021). PubMed: 33846884
- Jurivich DA et al. Multifactorial Attenuation of the Murine Heat Shock Response With Age. J Gerontol A Biol Sci Med Sci 75:1846-1852 (2020). PubMed: 31612204
- Pan X et al. Heat shock factor 1 suppresses the HIV-induced inflammatory response by inhibiting nuclear factor-?B. Cell Immunol 327:26-35 (2018). PubMed: 29525181
- Jin X et al. Modulation of Heat Shock Factor 1 Activity through Silencing of Ser303/Ser307 Phosphorylation Supports a Metabolic Program Leading to Age-Related Obesity and Insulin Resistance. Mol Cell Biol 38:N/A (2018). PubMed: 29941492
- Jin X et al. WITHDRAWN: Abrogation of heat shock factor 1 (Hsf1) phosphorylation deregulates its activity and lowers activation threshold, leading to obesity in mice. J Biol Chem N/A:N/A (2017). PubMed: 28724629
- Lee JH et al. Mitochondrial Respiratory Dysfunction Induces Claudin-1 Expression via Reactive Oxygen Species-mediated Heat Shock Factor 1 Activation, Leading to Hepatoma Cell Invasiveness. J Biol Chem 290:21421-31 (2015). PubMed: 26157141