Anti-HSF1 (phospho S320)抗体[EPR1712] (ab76183)


  • 产品名称Anti-HSF1 (phospho S320)抗体[EPR1712]
    参阅全部 HSF1 一抗
  • 描述
    兔单克隆抗体[EPR1712] to HSF1 (phospho S320)
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic phospho-peptide from residues surrounding serine 320 of human HSF1.

  • 阳性对照
    • HeLa cell lysate, human muscle tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.




Our Abpromise guarantee covers the use of ab76183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/20000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
    • 序列相似性Belongs to the HSF family.
    • 结构域the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
    • 翻译后修饰Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
      Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
    • 细胞定位Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Heat shock factor 1 antibody
      • Heat shock factor protein 1 antibody
      • Heat shock transcription factor 1 antibody
      • HSF 1 antibody
      • hsf1 antibody
      • HSF1_HUMAN antibody
      • HSTF 1 antibody
      • HSTF1 antibody
      see all

    Anti-HSF1 (phospho S320) antibody [EPR1712] 图像

    • Immunohistochemical analysis of paraffin-embedded human muscles using ab76183 at 1/100 dilution.
    • All lanes : Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183) at 1/20000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate, heat treated at 42°C

      Lysates/proteins at 10 µg per lane.

      Goat anti-rabbit-HRP at 1/1000 dilution
      developed using the ECL technique

      Predicted band size : 57 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

    Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183)参考文献

    This product has been referenced in:

    See all 2 Publications for this product

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