RabMAb

Anti-HSF1 (phospho S303 + S307)抗体[EP1711Y] (ab81281)

概述

  • 产品名称
    Anti-HSF1 (phospho S303 + S307)抗体[EP1711Y]
    参阅全部 HSF1 一抗
  • 描述
    兔单克隆抗体[EP1711Y] to HSF1 (phospho S303 + S307)
  • 特异性
    ab81281 antibody recognises HSF1 (phospho S303 + S307)
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • 阳性对照
    • HeLa cell lysates, Human breast carcinoma.
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab81281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/50000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能
      DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
    • 序列相似性
      Belongs to the HSF family.
    • 结构域
      the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
    • 翻译后修饰
      Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
      Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
    • 细胞定位
      Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Heat shock factor 1 antibody
      • Heat shock factor protein 1 antibody
      • Heat shock transcription factor 1 antibody
      • HSF 1 antibody
      • hsf1 antibody
      • HSF1_HUMAN antibody
      • HSTF 1 antibody
      • HSTF1 antibody
      see all

    Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] 图像

    • All lanes : Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281) at 1/50000 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate AP treated

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRp labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 57 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using 1/100 ab81281

    Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)参考文献

    This product has been referenced in:
    • Asano Y  et al. IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis. Sci Rep 6:19174 (2016). WB . Read more (PubMed: 26754925) »
    • Zhao J  et al. Correlation between the expression of Id-1 and hyperthermia-associated molecules in oral squamous cell carcinoma. J Clin Pathol 66:758-63 (2013). IHC ; Human . Read more (PubMed: 23723304) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us and bringing this to our attention.


    We have tested both products (ab76076 and ab81281) in mouse and rat samples, via WB.
    The samples we tested were brain, heart, kidney and spleen for each, in mouse and r...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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