Anti-HSF1 (phospho S303 + S307)抗体[EP1711Y] (ab81281)

概述

• 产品名称
  Anti-HSF1 (phospho S303 + S307)抗体[EP1711Y]
  参阅全部 HSF1 一抗
• 描述
  兔单克隆抗体[EP1711Y] to HSF1 (phospho S303 + S307)
• 特异性
  ab81281 antibody recognises HSF1 (phospho S303 + S307)
• 经测试应用
  适用于: WB, IHC-P, ICC/IFmore details
  不适用于: Flow Cyt or IP
• 种属反应性
  与反应: Human
  
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

• 阳性对照
  • HeLa cell lysates, Human breast carcinoma.
• 常规说明

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• 存储溶液
  pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA
• 纯度
  Tissue culture supernatant
• 克隆
  单克隆
• 克隆编号
  EP1711Y
• 同种型
  IgG
• 研究领域
  • Tags & Cell Markers
  • Subcellular Markers
  • Nucleus
  • Other Nuclear Bodies

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Cardiovascular
  • Heart
  • Cardiogenesis
  • Transcription factors/regulators

应用

靶标

• 功能
  DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
• 序列相似性
  Belongs to the HSF family.
• 结构域
  the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
• 翻译后修饰
  Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
  Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
• 细胞定位
  Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.

• Target information above from: UniProt accession Q00613 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 3297 Human
  • Omim: 140580 Human
  • SwissProt: Q00613 Human
  • Unigene: 530227 Human
• 别名
  • Heat shock factor 1 antibody
  • Heat shock factor protein 1 antibody
  • Heat shock transcription factor 1 antibody

  • HSF 1 antibody
  • hsf1 antibody
  • HSF1_HUMAN antibody
  • HSTF 1 antibody
  • HSTF1 antibody

  see all

实验方案

• Immunohistochemistry protocols
• Immunocytochemistry & immunofluorescence protocols
• Western blot protocols