概述

  • 产品名称Anti-HSF1抗体[J7F9]
    参阅全部 HSF1 一抗
  • 描述
    小鼠单克隆抗体[J7F9] to HSF1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human HSF1 (1-529 aa) purified from E. coli.

  • 阳性对照
    • Human Prostate tissue; Hela cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab115303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • 序列相似性Belongs to the HSF family.
  • 结构域the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 翻译后修饰Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heat shock factor 1 antibody
    • Heat shock factor protein 1 antibody
    • Heat shock transcription factor 1 antibody
    • HSF 1 antibody
    • hsf1 antibody
    • HSF1_HUMAN antibody
    • HSTF 1 antibody
    • HSTF1 antibody
    see all

Anti-HSF1 antibody [J7F9] 图像

  • ab115303, at 15 µg/ml, staining HSF1 in formalin-fixed, paraffin-embedded Human Prostate tissue by Immunohistochemistry, using a biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-HSF1 antibody [J7F9] (ab115303) at 1/1000 dilution + Hela cell lysate at 30 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 57 kDa

Anti-HSF1 antibody [J7F9] (ab115303)参考文献

This product has been referenced in:
  • Filone CM  et al. The master regulator of the cellular stress response (HSF1) is critical for orthopoxvirus infection. PLoS Pathog 10:e1003904 (2014). WB ; Human . Read more (PubMed: 24516381) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela cells)
Permeabilization Yes - 0.5% NP-40
Specification Hela cells
Blocking step Super block from Scy-tek as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 25 2015

Application Western blot
Sample Human Cell lysate - whole cell (HEK293 (kidney cell))
Gel Running Conditions Reduced Denaturing (4-12% Gradient Gel)
Loading amount 5 µg
Specification HEK293 (kidney cell)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Username

Dr. Sam Nowitzki

Verified customer

提交于 Jun 29 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Scytek Superblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Sample Human Cell (HEK203 (kidney))
Specification HEK203 (kidney)
Permeabilization Yes - Triton X-100 (0.1%)
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 31 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"