概述

  • 产品名称Anti-hSET1抗体- ChIP Grade
    参阅全部 hSET1 一抗
  • 描述
    兔多克隆抗体to hSET1 - ChIP Grade
  • 经测试应用适用于: IHC-P, ChIP, ChIP/Chip, WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide mapping to a region between residues 1200 and 1250 of human hSET1 (TrEMBL entry O15047)

  • 阳性对照
    • 293T whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab70378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIP Use 1-5 µg for µg of chromatin.
ChIP/Chip Use 10 µg for µg of chromatin.
WB 1/1000 - 1/10000. Predicted molecular weight: 186 kDa.
IP Use at 1-4 µg/mg of lysate.
ICC/IF 1/400. Use with paraformaldehyde or methanol-fixed cells.

靶标

  • 功能Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
  • 序列相似性Contains 1 post-SET domain.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SET domain.
  • 细胞定位Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Histone-lysine N-methyltransferase SETD1A antibody
    • hSET1A antibody
    • KMT2F antibody
    • Lysine N methyltransferase 2F antibody
    • Lysine N-methyltransferase 2F antibody
    • SET domain containing 1A antibody
    • SET domain containing protein 1A antibody
    • SET domain-containing protein 1A antibody
    • SET1 antibody
    • Set1 Ash2 histone methyltransferase complex subunit SET1 antibody
    • Set1/Ash2 histone methyltransferase complex subunit SET1 antibody
    • SET1A antibody
    • SET1A_HUMAN antibody
    • SETD1A antibody
    see all

Anti-hSET1 antibody - ChIP Grade 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling hSET1 with ab70378 at 1/1000 (1µg/ml). Detection: DAB.
  • Lanes 1 - 4 : Anti-hSET1 antibody - ChIP Grade (ab70378) at 1 µg/ml
    Lanes 5 - 8 : Anti-hSET1 antibody - ChIP Grade (ab70378) at 0.2 µg/ml

    Lane 1 : 293T whole cell lysate at 100 µg
    Lane 2 : 293T whole cell lysate at 200 µg
    Lane 3 : 293T nuclear extract at 100 µg
    Lane 4 : 293T nuclear extract at 200 µg
    Lane 5 : 293T whole cell lysate at 100 µg
    Lane 6 : 293T whole cell lysate at 200 µg
    Lane 7 : 293T nuclear extract at 100 µg
    Lane 8 : 293T nuclear extract at 200 µg

    Developed using the ECL technique

    Predicted band size : 186 kDa
  • Ab70378 (10 µg) was used to immunoprecipitate chromatin from K562 cells. Immunoprecipitated and reference DNA were amplified by ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray and analyzed. As a control a similar experiment was performed using normal rabbit IgG.
  • ab70378 (1/400) staining hSET1 in assynchronous HeLa cells (green). Cells were paraformaldehyde-fixed, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview

Anti-hSET1 antibody - ChIP Grade (ab70378)参考文献

This product has been referenced in:
  • Hopkin AS  et al. GRHL3/GET1 and trithorax group members collaborate to activate the epidermal progenitor differentiation program. PLoS Genet 8:e1002829 (2012). WB, ChIP ; Human . Read more (PubMed: 22829784) »
  • Le Douce V  et al. LSD1 cooperates with CTIP2 to promote HIV-1 transcriptional silencing. Nucleic Acids Res 40:1904-15 (2012). ChIP ; Human . Read more (PubMed: 22067449) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Gonad)
Antigen retrieval step Enzymatic
Permeabilization No
Specification Gonad
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (gonadotrope)
Loading amount 20 µg
Specification gonadotrope
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Andrea Wijeweera

Verified customer

提交于 Nov 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

提交于 Aug 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"