概述

  • 产品名称Anti-hSET1抗体
    参阅全部 hSET1 一抗
  • 描述
    兔多克隆抗体to hSET1
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide mapping to a region between residue 1675 and the C-terminus (residue 1709) of human hSET1.

  • 阳性对照
    • 293T whole cell lysate or nuclear extracts

性能

应用

Our Abpromise guarantee covers the use of ab70379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 186 kDa.
IP Use at 1-4 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration. PubMed: 22067449

靶标

  • 功能Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
  • 序列相似性Contains 1 post-SET domain.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SET domain.
  • 细胞定位Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Histone-lysine N-methyltransferase SETD1A antibody
    • hSET1A antibody
    • KMT2F antibody
    • Lysine N methyltransferase 2F antibody
    • Lysine N-methyltransferase 2F antibody
    • SET domain containing 1A antibody
    • SET domain containing protein 1A antibody
    • SET domain-containing protein 1A antibody
    • SET1 antibody
    • Set1 Ash2 histone methyltransferase complex subunit SET1 antibody
    • Set1/Ash2 histone methyltransferase complex subunit SET1 antibody
    • SET1A antibody
    • SET1A_HUMAN antibody
    • SETD1A antibody
    see all

Anti-hSET1 antibody 图像

  • Lanes 1 - 4 : Anti-hSET1 antibody (ab70379) at 1 µg/ml
    Lanes 5 - 8 : Anti-hSET1 antibody (ab70379) at 0.2 µg/ml

    Lane 1 : 293T whole cell lysate at 100 µg
    Lane 2 : 293T whole cell lysate at 200 µg
    Lane 3 : 293T nuclear lysate at 100 µg
    Lane 4 : 293T nuclear lysate at 200 µg
    Lane 5 : 293T whole cell lysate at 100 µg
    Lane 6 : 293T whole cell lysate at 200 µg
    Lane 7 : 293T nuclear lysate at 100 µg
    Lane 8 : 293T nuclear lysate at 200 µg

    Developed using the ECL technique

    Predicted band size : 186 kDa
    Observed band size : >250 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 seconds
  • Detection of Human hSET1 by Immunoprecipitation. Control IgG used in lane 1, ab70379 used at 5 µg/5mg nuclear extract from 293T cells in lane 2. Immunoprecipitated hSET1 was detected with post IP WB using ab70379 at 0.2 µg/ml. Detection: Chemiluminescence.

Anti-hSET1 antibody (ab70379)参考文献

This product has been referenced in:
  • Le Douce V  et al. LSD1 cooperates with CTIP2 to promote HIV-1 transcriptional silencing. Nucleic Acids Res 40:1904-15 (2012). ICC/IF, ChIP . Read more (PubMed: 22067449) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (NIH3T3)
Loading amount 40 µg
Specification NIH3T3
Gel Running Conditions Reduced Denaturing (6%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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提交于 Apr 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"