Recombinant protein fragment with a sequence corresponding to a region within amino acids 1-187 of Human HSD3a (NP_001809).
HeLa and HepG2 cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
1/100 - 1/250.
Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20-alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route.
Belongs to the aldo/keto reductase family.
The N-terminus is blocked.
Information by UniProt
3 alpha HSD1 antibody
3-alpha-hydroxysteroid dehydrogenase type I antibody
Western blot - Anti-HSD3a antibody (ab96135)
Anti-HSD3a antibody (ab96135) at 1/1000 dilution (10% SDS PAGE) + HeLa whole cell lysate at 30 µg
Predicted band size: 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD3a antibody (ab96135)
ab96135, at 1/100, staining paraffin-embedded gastric CA by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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