Anti-HSD17B2抗体(ab103665)

概述

  • 产品名称Anti-HSD17B2抗体
    参阅全部 HSD17B2 一抗
  • 描述
    兔多克隆抗体to HSD17B2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 270-300 of Human HSD17B2

  • 阳性对照
    • MDA-MB435 cell line lysates, Human prostate carcinoma

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab103665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.
  • 序列相似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 17 beta HSD 2 antibody
    • 17-beta-HSD 2 antibody
    • 17-beta-hydroxysteroid dehydrogenase type 2 antibody
    • 20 alpha HSD antibody
    • 20 alpha hydroxysteroid dehydrogenase antibody
    • 20 alpha-hydroxysteroid dehydrogenase antibody
    • 20-alpha-HSD antibody
    • DHB2_HUMAN antibody
    • E2DH antibody
    • EDH17B2 antibody
    • Estradiol 17-beta-dehydrogenase 2 antibody
    • HSD17 antibody
    • Hsd17b2 antibody
    • Hydroxysteroid (17 beta) dehydrogenase 2 antibody
    • Microsomal 17 beta hydroxysteroid dehydrogenase antibody
    • Microsomal 17-beta-hydroxysteroid dehydrogenase antibody
    • SDR9C2 antibody
    • Short chain dehydrogenase/reductase family 9C, member 2 antibody
    • Testosterone 17 beta dehydrogenase antibody
    • Testosterone 17-beta-dehydrogenase antibody
    see all

Anti-HSD17B2 antibody 图像

  • Anti-HSD17B2 antibody (ab103665) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

    Predicted band size : 43 kDa
  • IHC analysis of HSD17B2 expression in formalin fixed and paraffin embedded Human prostate carcinoma using 1/50 ab103665 followed by peroxidase conjugation of the secondary antibody and DAB staining.

Anti-HSD17B2 antibody (ab103665)参考文献

ab103665 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Cow Tissue lysate - other (Corpus luteum)
Specification Corpus luteum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Msano

Verified customer

提交于 Jul 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"