Anti-HSD17B2抗体(ab103665)

概述

  • 产品名称
    Anti-HSD17B2抗体
    参阅全部 HSD17B2 一抗
  • 描述
    兔多克隆抗体to HSD17B2
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 270-300 of Human HSD17B2

  • 阳性对照
    • MDA-MB435 cell line lysates, Human prostate carcinoma

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab103665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.
  • 序列相似性
    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 17 beta HSD 2 antibody
    • 17-beta-HSD 2 antibody
    • 17-beta-hydroxysteroid dehydrogenase type 2 antibody
    • 20 alpha HSD antibody
    • 20 alpha hydroxysteroid dehydrogenase antibody
    • 20 alpha-hydroxysteroid dehydrogenase antibody
    • 20-alpha-HSD antibody
    • DHB2_HUMAN antibody
    • E2DH antibody
    • EDH17B2 antibody
    • Estradiol 17-beta-dehydrogenase 2 antibody
    • HSD17 antibody
    • Hsd17b2 antibody
    • Hydroxysteroid (17 beta) dehydrogenase 2 antibody
    • Microsomal 17 beta hydroxysteroid dehydrogenase antibody
    • Microsomal 17-beta-hydroxysteroid dehydrogenase antibody
    • SDR9C2 antibody
    • Short chain dehydrogenase/reductase family 9C, member 2 antibody
    • Testosterone 17 beta dehydrogenase antibody
    • Testosterone 17-beta-dehydrogenase antibody
    see all

图片

  • Anti-HSD17B2 antibody (ab103665) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

    Predicted band size : 43 kDa
  • IHC analysis of HSD17B2 expression in formalin fixed and paraffin embedded Human prostate carcinoma using 1/50 ab103665 followed by peroxidase conjugation of the secondary antibody and DAB staining.

文献

ab103665 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Cow Tissue lysate - other (Corpus luteum)
Specification
Corpus luteum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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提交于 Jul 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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