Anti-HSD17B14抗体(ab87011)

概述

  • 产品名称Anti-HSD17B14抗体
    参阅全部 HSD17B14 一抗
  • 描述
    兔多克隆抗体to HSD17B14
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 221-270 (PAEVGAAAVF LASEANFCTG IELLVTGGAE LGYGCKASRS TPVDAPDIPS) of Human HSD17B14 (NP_057330).

  • 阳性对照
    • Human fetal liver lysate.

性能

应用

Our Abpromise guarantee covers the use of ab87011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性HSD17B14 belongs to the short-chain dehydrogenases/reductases (SDR) family. It has NAD-dependent 17-beta-hydroxysteroid dehydrogenase activity and converts oestradiol to oestrone. The physiological substrate is not known. HSD17B14 acts on oestradiol and 5-androstene-3-beta,17-beta-diol (in vitro). It is highly expressed in brain, placenta, liver and kidney.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • 17 beta HSD 14 antibody
    • 17 beta hydroxysteroid dehydrogenase 14 antibody
    • 17 beta hydroxysteroid dehydrogenase DHRS10 antibody
    • Dehydrogenase/reductase (SDR family) member 10 antibody
    • Dehydrogenase/reductase SDR family member 10 antibody
    • DHRS10 antibody
    • Hydroxysteroid (17 beta) dehydrogenase 14 antibody
    • Retinal short chain dehydrogenase/reductase 3 antibody
    • Retinal short chain dehydrogenase/reductase retSDR3 antibody
    • retSDR3 antibody
    • SDR3 antibody
    • SDR47C1 antibody
    • Short chain dehydrogenase/reductase family 47C, member 1 antibody
    see all

Anti-HSD17B14 antibody 图像

  • Anti-HSD17B14 antibody (ab87011) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of Human bladder tissue, staining HSD17B14 with ab87011.

    Tissue was fixed with formaldehyde and blocked with 1% BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/25 in TEG) for 1 hour at 22 °C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

Anti-HSD17B14 antibody (ab87011)参考文献

ab87011 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Bladder cancer)
Specification Bladder cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Niels Fristrup

Verified customer

提交于 Dec 04 2012

DISCOUNT CODE: ABTRIAL-EB3TS
Expiration date: 10-01-2013
Value: Euro 410

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