Anti-HSD17B14抗体(ab87011)

概述

  • 产品名称
    Anti-HSD17B14抗体
    参阅全部 HSD17B14 一抗
  • 描述
    兔多克隆抗体to HSD17B14
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 221-270 (PAEVGAAAVF LASEANFCTG IELLVTGGAE LGYGCKASRS TPVDAPDIPS) of Human HSD17B14 (NP_057330).

  • 阳性对照
    • Human fetal liver lysate.

性能

应用

Our Abpromise guarantee covers the use of ab87011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性
    HSD17B14 belongs to the short-chain dehydrogenases/reductases (SDR) family. It has NAD-dependent 17-beta-hydroxysteroid dehydrogenase activity and converts oestradiol to oestrone. The physiological substrate is not known. HSD17B14 acts on oestradiol and 5-androstene-3-beta,17-beta-diol (in vitro). It is highly expressed in brain, placenta, liver and kidney.
  • 细胞定位
    Cytoplasmic
  • 数据库链接
  • 别名
    • 17 beta HSD 14 antibody
    • 17 beta hydroxysteroid dehydrogenase 14 antibody
    • 17 beta hydroxysteroid dehydrogenase DHRS10 antibody
    • Dehydrogenase/reductase (SDR family) member 10 antibody
    • Dehydrogenase/reductase SDR family member 10 antibody
    • DHRS10 antibody
    • Hydroxysteroid (17 beta) dehydrogenase 14 antibody
    • Retinal short chain dehydrogenase/reductase 3 antibody
    • Retinal short chain dehydrogenase/reductase retSDR3 antibody
    • retSDR3 antibody
    • SDR3 antibody
    • SDR47C1 antibody
    • Short chain dehydrogenase/reductase family 47C, member 1 antibody
    see all

图片

  • Anti-HSD17B14 antibody (ab87011) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of Human bladder tissue, staining HSD17B14 with ab87011.

    Tissue was fixed with formaldehyde and blocked with 1% BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/25 in TEG) for 1 hour at 22 °C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

文献

ab87011 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Bladder cancer)
Specification
Bladder cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Niels Fristrup

Verified customer

提交于 Dec 04 2012

DISCOUNT CODE: ABTRIAL-EB3TS
Expiration date: 10-01-2013
Value: Euro 410

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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