概述

  • 产品名称Anti-HSD11B2抗体
    参阅全部 HSD11B2 一抗
  • 描述
    兔多克隆抗体to HSD11B2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Pig
  • 免疫原

    Synthetic peptide designed within amino acids: MERWPWPSGG AWLLVAARAL LQLLRSDLRL GRPLLAALAL LAALDWLCQR, corresponding to N terminal residues 1-50 of Human HSD11B2 (NP_000187)

  • 阳性对照
    • Fetal muscle lysate.

性能

应用

Our Abpromise guarantee covers the use of ab80317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
  • 组织特异性Found in placenta, kidney, pancreas, prostate, ovary, small intestine and colon.
  • 疾病相关Defects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME) [MIM:218030]. AME is a potentially fatal disease characterized by severe juvenile low-renin hypertension, sodium retention, hypokalemia and low levels of aldosterone. It often leads to nephrocalcinosis.
  • 序列相似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • 细胞定位Microsome. Endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 11 beta HSD2 antibody
    • 11 beta hydroxysteroid dehydrogenase type 2 antibody
    • 11 DH2 antibody
    • 11-beta-HSD2 antibody
    • 11-beta-hydroxysteroid dehydrogenase type 2 antibody
    • 11-DH2 antibody
    • AME antibody
    • AME1 antibody
    • Corticosteroid 11 beta dehydrogenase isozyme 2 antibody
    • Corticosteroid 11-beta-dehydrogenase isozyme 2 antibody
    • DHI2_HUMAN antibody
    • HSD11B2 antibody
    • HSD11K antibody
    • HSD2 antibody
    • Hydroxysteroid 11 beta dehydrogenase 2 antibody
    • Hydroxysteroid 11 beta dehydrogenase isoenzyme 2 antibody
    • NAD dependent 11 beta hydroxysteroid dehydrogenase antibody
    • NAD-dependent 11-beta-hydroxysteroid dehydrogenase antibody
    • SDR9C3 antibody
    • Short chain dehydrogenase/reductase family 9C, member 3 antibody
    see all

Anti-HSD11B2 antibody 图像

  • Anti-HSD11B2 antibody (ab80317) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

Anti-HSD11B2 antibody (ab80317)参考文献

This product has been referenced in:
  • Cuffe JS  et al. Mid- to late term hypoxia in the mouse alters placental morphology, glucocorticoid regulatory pathways and nutrient transporters in a sex-specific manner. J Physiol N/A:N/A (2014). Read more (PubMed: 24801305) »
  • Kuroda K  et al. Induction of 11ß-HSD 1 and activation of distinct mineralocorticoid receptor- and glucocorticoid receptor-dependent gene networks in decidualizing human endometrial stromal cells. Mol Endocrinol 27:192-202 (2013). Human . Read more (PubMed: 23275455) »

See all 5 Publications for this product

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (Prostate Cell Lines)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 20 µg
Specification Prostate Cell Lines
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Abcam user community

Verified customer

提交于 Sep 29 2015

Application Western blot
Loading amount 8 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% Tris/Glycine-SDS)
Sample Sheep Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Rob Cordery-Cotter

Verified customer

提交于 Nov 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"