The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
组织特异性Found in placenta, kidney, pancreas, prostate, ovary, small intestine and colon.
疾病相关Defects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME) [MIM:218030]. AME is a potentially fatal disease characterized by severe juvenile low-renin hypertension, sodium retention, hypokalemia and low levels of aldosterone. It often leads to nephrocalcinosis.
序列相似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Short chain dehydrogenase/reductase family 9C, member 3 antibody
Anti-HSD11B2 antibody 图像
Western blot - HSD11B2 antibody (ab80317)
Anti-HSD11B2 antibody (ab80317) at 1 µg/ml + fetal muscle lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 44 kDa Observed band size : 44 kDa Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
Anti-HSD11B2 antibody (ab80317)参考文献
This product has been referenced in:
Cuffe JS et al. Mid- to late term hypoxia in the mouse alters placental morphology, glucocorticoid regulatory pathways and nutrient transporters in a sex-specific manner. J PhysiolN/A:N/A (2014).
Read more (PubMed: 24801305) »
Kuroda K et al. Induction of 11ß-HSD 1 and activation of distinct mineralocorticoid receptor- and glucocorticoid receptor-dependent gene networks in decidualizing human endometrial stromal cells. Mol Endocrinol27:192-202 (2013).
Read more (PubMed: 23275455) »