概述

  • 产品名称Anti-HSD11B1抗体
    参阅全部 HSD11B1 一抗
  • 描述
    兔多克隆抗体to HSD11B1
  • 经测试应用适用于: WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Horse, Human
    预测可用于: Rabbit, Hamster, Cow, Cat, Dog, Monkey, Cynomolgus Monkey, Macaque Monkey
  • 免疫原

    Synthetic peptide:

    CLELGAASAHYIAGT

    , corresponding to amino acids 78/92 of Human HSD11B1

  • 阳性对照
    • Rat and mouse liver microsomes.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab39364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).Can be blocked with HSD11B1 peptide (ab101097).
IHC-P Use a concentration of 2 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 20385225

靶标

  • 功能Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
  • 组织特异性Widely expressed. Highest expression in liver.
  • 疾病相关Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
  • 序列相似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • 翻译后修饰Glycosylated.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 11 beta HSD 1 antibody
    • 11 beta HSD1 antibody
    • 11 beta hydroxysteroid dehydrogenase 1 antibody
    • 11 DH antibody
    • 11-beta hydroxysteroid dehydrogenase, type 1 antibody
    • 11-beta-HSD1 antibody
    • 11-beta-hydroxysteroid dehydrogenase 1 antibody
    • 11-DH antibody
    • 11DH antibody
    • Corticosteroid 11 beta dehydrogenase isozyme 1 antibody
    • Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody
    • CORTRD2 antibody
    • DHI1_HUMAN antibody
    • HDL antibody
    • HSD 11 antibody
    • HSD11 antibody
    • HSD11B antibody
    • HSD11B1 antibody
    • HSD11L antibody
    • Hydroxysteroid (11 beta) dehydrogenase antibody
    • Hydroxysteroid (11 beta) dehydrogenase 1 antibody
    • MGC13539 antibody
    • SDR26C1 antibody
    • short chain dehydrogenase/reductase family 26C, member 1 antibody
    see all

Anti-HSD11B1 antibody 图像


  • Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Rob Cordery-Cotter. Experimental work performed by Megan A. Walton.

    Western blot analysis of Sheep liver tissue lysate (5µg/lane) labeling HSD11B1 with ab39364 at 1/500 (16 hours at 4ºC). An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-HSD11B1 antibody (ab39364) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa


    Exposure time : 16 minutes
    HSD11B1 contains a number of potential glycosylation sites (SwissProt) which may explain the higher migration pattern observed.

Anti-HSD11B1 antibody (ab39364)参考文献

This product has been referenced in:
  • Zeng X  et al. Lysine-specific demethylase 1 promotes brown adipose tissue thermogenesis via repressing glucocorticoid activation. Genes Dev 30:1822-36 (2016). Read more (PubMed: 27566776) »
  • Jacob W  et al. Decreased adipogenesis and adipose tissue in mice with inactivated protein phosphatase 5. Biochem J 466:163-76 (2015). Read more (PubMed: 25437352) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris ⁄EDTA
Sample Horse Tissue sections (Testicular tissue)
Specification Testicular tissue
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 May 30 2014

Application Western blot
Loading amount 5 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Sample Sheep Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Rob Cordery-Cotter

Verified customer

提交于 Nov 12 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Reviewing theimage, despite the nonspecific bands there is a well resolved band at the expected molecular weight and it is odd that the blocking peptide did not complete obliterate this band as it ha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"