概述

  • 产品名称Anti-HSD11B1抗体
    参阅全部 HSD11B1 一抗
  • 描述
    山羊多克隆抗体to HSD11B1
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Sheep, Cow, Plasmodium falciparum
  • 免疫原

    Synthetic peptide:

    CTSYNMDRFINK

    from the C terminus of Human HSD11B1. (Peptide available as ab136288.)

  • 阳性对照
    • Human Liver lysate for Western blot.

性能

应用

Our Abpromise guarantee covers the use of ab27501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA 1/4000.
WB Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).

靶标

  • 功能Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
  • 组织特异性Widely expressed. Highest expression in liver.
  • 疾病相关Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
  • 序列相似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • 翻译后修饰Glycosylated.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 11 beta HSD 1 antibody
    • 11 beta HSD1 antibody
    • 11 beta hydroxysteroid dehydrogenase 1 antibody
    • 11 DH antibody
    • 11-beta hydroxysteroid dehydrogenase, type 1 antibody
    • 11-beta-HSD1 antibody
    • 11-beta-hydroxysteroid dehydrogenase 1 antibody
    • 11-DH antibody
    • 11DH antibody
    • Corticosteroid 11 beta dehydrogenase isozyme 1 antibody
    • Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody
    • CORTRD2 antibody
    • DHI1_HUMAN antibody
    • HDL antibody
    • HSD 11 antibody
    • HSD11 antibody
    • HSD11B antibody
    • HSD11B1 antibody
    • HSD11L antibody
    • Hydroxysteroid (11 beta) dehydrogenase antibody
    • Hydroxysteroid (11 beta) dehydrogenase 1 antibody
    • MGC13539 antibody
    • SDR26C1 antibody
    • short chain dehydrogenase/reductase family 26C, member 1 antibody
    see all

Anti-HSD11B1 antibody 图像

  • Anti-HSD11B1 antibody (ab27501) at 0.3 µg/ml + Human Liver lysate (35 µg protein in RIPA buffer).
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab27501 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27501, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-HSD11B1 antibody (ab27501)参考文献

This product has been referenced in:
  • Chinetti-Gbaguidi G  et al. Peroxisome proliferator-activated receptor-? activation induces 11ß-hydroxysteroid dehydrogenase type 1 activity in human alternative macrophages. Arterioscler Thromb Vasc Biol 32:677-85 (2012). WB ; Human . Read more (PubMed: 22207732) »

See 1 Publication for this product

Product Wall

Vielen Dank für Ihren Anruf.

Ich freue mich, Ihnen mitteilen zu können, dass das Blocking -Peptid für ab27501 nun in den Katalog aufgenommen wurde.

Die Artikelnummer ist ab136288 und das datenblatt kann über folg...

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Thank you for your interesting enquiry. The immunogen for this antibody (ab27501) is TSYNMDRFINK, from the C Terminus of the protein sequence according to NP_005516; NP_861420. You are correct, I have had a look on the NCBI protein data base via PubMed...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"