antigen, corresponding to amino acids 160-232 (QFLIEIMEIN EKLAEAESEA AMKEIESIVK AKQKEFTDNV SSAFEQDDFE EAKEILTKMR YFSNIEEKIK LKK) of Human HSCB.
Human colon tissue; Transfected HEK293T Over-expression Lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1000 - 1/2500.
May act as a co-chaperone in iron-sulfur cluster assembly in mitochondria.
Liver, muscle and heart.
Cofactor biosynthesis; iron-sulfur cluster biosynthesis.
Belongs to the hscB family.
Contains 1 J domain.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-HSCB antibody (ab121569)
Immunofluorescent staining of Human cell line U-251MG shows positivity in plasma membrane, cytoplasm and centrosome. Recommended concentration of ab121569 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSCB antibody (ab121569)
ab121569 at 1/450 dilution staining HSCB in Paraffin-embedded Human Colon tissue by Immunohistochemistry.
Western blot - Anti-HSCB antibody (ab121569)
All lanes : Anti-HSCB antibody (ab121569) at 1/250 dilution Lane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : HSCB transfected HEK293T Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)) Developed using the ECL technique.
has not yet been referenced specifically in any publications.
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