RabMAb

Anti-HSC70 Interacting Protein HIP抗体[EP3264] (ab76005)

概述

  • 产品名称Anti-HSC70 Interacting Protein HIP抗体[EP3264]
    参阅全部 HSC70 Interacting Protein HIP 一抗
  • 描述
    兔单克隆抗体[EP3264] to HSC70 Interacting Protein HIP
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminus of human HSC70 Interacting Protein HIP.

  • 阳性对照
    • 293T and SW480 cell lysates; human colonic adenocarcinoma and normal colon tissues.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EP3264
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2500 - 1/5000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins.
    • 序列相似性Belongs to the FAM10 family.
      Contains 1 STI1 domain.
      Contains 3 TPR repeats.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AAG 2 antibody
      • AAG2 antibody
      • Aging associated protein 2 antibody
      • F10A1_HUMAN antibody
      • FAM10A1 antibody
      • FAM10A4 antibody
      • FLJ27260 antibody
      • Heat shock 70kD protein binding protein antibody
      • Hip antibody
      • HOP antibody
      • Hsc70 interacting protein antibody
      • Hsc70-interacting protein antibody
      • Hsp70 interacting protein antibody
      • HSPABP 1 antibody
      • HSPABP antibody
      • HSPABP1 antibody
      • MGC129952 antibody
      • OTTHUMP00000028873 antibody
      • P48 antibody
      • PRO0786 antibody
      • Progesterone receptor associated p48 protein antibody
      • Progesterone receptor-associated p48 protein antibody
      • Protein FAM10A1 antibody
      • Putative tumor suppressor ST13 antibody
      • Renal carcinoma antigen NY REN 33 antibody
      • Renal carcinoma antigen NY-REN-33 antibody
      • SNC 6 antibody
      • SNC6 antibody
      • ST 13 antibody
      • St13 antibody
      • Suppression of tumorigenicity 13 antibody
      • Suppression of tumorigenicity 13 (colon carcinoma) antibody
      • Suppression of tumorigenicity 13 protein antibody
      • Suppression of tumorigenicity protein 13 antibody
      see all

    Anti-HSC70 Interacting Protein HIP antibody [EP3264] 图像

    • All lanes : Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005) at 1/100000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/1000 dilution
      Developed using the ECL technique

      Predicted band size : 41 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    • ab76005 at 1/100 dilution staining HSC70 Interacting Protein HIP in human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • ab76005 at 1/100 dilution staining HSC70 Interacting Protein HIP in human normal colon by Immunohistochemistry, Paraffin-embedded tissue.

    Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005)参考文献

    ab76005 has not yet been referenced specifically in any publications.

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