概述

  • 产品名称Anti-Hsc70抗体
    参阅全部 Hsc70 一抗
  • 描述
    兔多克隆抗体to Hsc70
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Goat, Horse, Dog, Saccharomyces cerevisiae, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 143-192 (VVTVPAYFND SQRQATKDAG TIAGLNVLRI INEPTAAAIA YGLDKKVGAE) of Human Hsc70 (NP_006588).

  • 阳性对照
    • COLO205 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab98156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/500.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the heat shock protein 70 family.
  • 结构域The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • 细胞定位Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2410008N15Rik antibody
    • Constitutive heat shock protein 70 antibody
    • Epididymis luminal protein 33 antibody
    • Epididymis secretory sperm binding protein Li 72p antibody
    • Heat shock 70 kDa protein 8 antibody
    • Heat shock 70kD protein 10 antibody
    • Heat shock 70kD protein 8 antibody
    • Heat shock 70kDa protein 8 antibody
    • Heat shock cognate 71 kDa protein antibody
    • Heat shock cognate protein 54 antibody
    • Heat shock cognate protein 71 kDa antibody
    • Heat shock protein 8 antibody
    • Heat shock protein A8 antibody
    • Heat-shock70-KD protein 10, formerly antibody
    • HEL 33 antibody
    • HEL S 72p antibody
    • HSC54 antibody
    • HSC71 antibody
    • Hsc73 antibody
    • HSP71 antibody
    • HSP73 antibody
    • HSP7C_HUMAN antibody
    • HSPA10 antibody
    • HSPA8 antibody
    • LAP1 antibody
    • Lipopolysaccharide associated protein 1 antibody
    • LPS associated protein 1 antibody
    • LPS associated protein antibody
    • MGC102007 antibody
    • MGC106514 antibody
    • MGC114311 antibody
    • MGC118485 antibody
    • MGC131511 antibody
    • MGC29929 antibody
    • N-myristoyltransferase inhibitor protein 71 antibody
    • NIP71 antibody
    see all

Anti-Hsc70 antibody 图像

  • Anti-HSPA8 / HSC70 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed/paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
  • Anti-HSPA8 / HSC70 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed/paraffin-embedded tissue after heat-induced antigen retrieval. Antibodyconcentration 5 ug/ml.
  • Application: IHC. Species+tissue/cell type: Human brain stem cells. Primary antibody dilution: 1:500. Secondary antibody: Goat anti-rabbit Alexa-Fluor 594. Secondary antibody dilution: 1:1000
  • Anti-Hsc70 antibody (ab98156) at 1 µg/ml (in 5% skim milk / PBS buffer) + COLO205 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 71 kDa
    Gel concentration: 12%
  • All lanes : Anti-Hsc70 antibody (ab98156) at 1/1000 dilution

    Lane 1 : Lysates prepared from Lamprey CNS.
    Lane 2 : Lysates prepared from rat brain.


    Predicted band size : 71 kDa

Anti-Hsc70 antibody (ab98156)参考文献

ab98156 has not yet been referenced specifically in any publications.

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