This antibody gave a positive signal in Recombinant Hsc70 protein as well as Human Breast and Human Kidney tissue lysates and the following whole cell lysates: MCF7; MDA MB 231; HEK293.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa).
功能Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
序列相似性Belongs to the heat shock protein 70 family.
结构域The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR. ISGylated.
细胞定位Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
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Anti-Hsc70 antibody 图像
Western blot - Anti-Hsc70 antibody (ab112549)
All lanes : Anti-Hsc70 antibody (ab112549) at 1 µg/ml
Lane 1 : Recombinant Protein:Hsc70 (Active) Lane 2 : Human breast tissue lysate - total protein (ab30090) Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 4 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 5 : Human kidney tissue lysate - total protein (ab30203) Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique